Excipients activate peroxidases in specific but not in non-specific reactions in organic solvents

被引：3
|
作者：
Xie, YC [1 ]
Das, PK [1 ]
Klibanov, AM [1 ]
机构：
[1] MIT, Dept Chem, Cambridge, MA 02139 USA
来源：
BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 18期
基金：
美国国家科学基金会;
关键词：
expoxidations; lyophilization; nonaqueous biocatalysis; peroxidase; sulfoxidations;
D O I：
10.1023/A:1011641622960
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Co-lyophilization with ligands and lyoprotectants markedly activated horseradish and soybean peroxidases in the oxidation of guaiacol in organic solvents (Dai & Klibanov (1999) Proc. Natl. Acad. Sci. USA 96: 9475-9478). However, such excipients gave little or no activation of these enzymes in non-specific reactions, sulfoxidation of thioanisole and epoxidation of styrene, presumably because they do not require a precise fine tuning of the enzyme active site.
引用
收藏
页码：1451 / 1454
页数：4
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