Post-translational modifications and their biological functions: Proteomic analysis and systematic approaches

被引：1

作者：

Seo, J

Lee, KJ ^{[1
]}

机构：

[1] Ewha Womans Univ, Ctr Cell Signaling Res, Seoul 120750, South Korea

[2] Ewha Womans Univ, Div Mol Life Sci, Seoul 120750, South Korea

[3] Ewha Womans Univ, Coll Pharm, Seoul 120750, South Korea

来源：

JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2004年 / 37卷 / 01期

关键词：

biological function; post-translational modifications; proteomics; mass spectrometry; systemic analysis;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently produced information on post-translational modifications makes it possible to interpret their biological regulation with new insights. Various protein modifications finely tune the cellular functions of each protein. Understanding the relationship between post-translational modifications. and functional changes ("post-translatomics") is another enormous project, not unlike the human genome project. Proteomics, combined with separation technology and mass spectrometry, makes it possible to dissect and characterize the individual parts of post-translational modifications and provide a systemic analysis. Systemic analysis of post-translational modifications in various signaling pathways has been applied to illustrate the kinetics of modifications. Availability will advance new technologies that improve sensitivity and peptide coverage. The progress of "post-translatomics", novel analytical technologies that are rapidly emerging, offer a great potential for determining the details of the modification sites.

引用

页码：35 / 44

页数：10

共 52 条

[1] Electrospray ionization and matrix assisted laser desorption/ionization mass spectrometry: Powerful analytical tools in recombinant protein chemistry [J].