A synthetic three-dimensional niche system facilitates generation of functional hematopoietic cells from human-induced pluripotent stem cells

被引：14

作者：

Xu, Yulin ^{[1
]}

Shan, Wei ^{[1
]}

Li, Xia ^{[1
]}

Wang, Binsheng ^{[1
]}

Liu, Senquan ^{[1
]}

Wang, Yebo ^{[1
]}

Long, Yan ^{[1
]}

Tie, Ruxiu ^{[1
]}

Wang, Limengmeng ^{[1
]}

Cai, Shuyang ^{[1
]}

Zhang, Hao ^{[1
]}

Lin, Yu ^{[1
]}

Zhang, Mingming ^{[1
]}

Zheng, Weiyan ^{[1
]}

Luo, Yi ^{[1
]}

Yu, Xiaohong ^{[1
]}

Yee, Jiing-Kuan ^{[2
]}

Ji, Junfeng ^{[3
]}

Huang, He ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Bone Marrow Transplantat Ctr, Affiliated Hosp 1, Hangzhou 310012, Zhejiang, Peoples R China

[2] City Hope Natl Med Ctr, Dept Diabet & Metab Dis Res, Duarte, CA 91010 USA

[3] Zhejiang Univ, Sch Med, Ctr Stem Cell & Regenerat Med, Hangzhou 310012, Zhejiang, Peoples R China

来源：

JOURNAL OF HEMATOLOGY & ONCOLOGY | 2016年 / 9卷

关键词：

Induced pluripotent stem cells; Hematopoietic stem cells; Niche; Three dimension; Transplantation; PROGENITOR CELLS; STROMAL CELLS; DIFFERENTIATION; MOUSE; SPECIFICATION; TRANSPLANTATION; FIBROBLASTS; TECHNOLOGY; COMMITMENT; INDUCTION;

D O I：

10.1186/s13045-016-0326-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: The efficient generation of hematopoietic stem cells (HSCs) from human-induced pluripotent stem cells (iPSCs) holds great promise in personalized transplantation therapies. However, the derivation of functional and transplantable HSCs from iPSCs has had very limited success thus far. Methods: We developed a synthetic 3D hematopoietic niche system comprising nanofibers seeded with bone marrow (BM)-derived stromal cells and growth factors to induce functional hematopoietic cells from human iPSCs in vitro. Results: Approximately 70 % of human CD34(+) hematopoietic cells accompanied with CD43(+) progenitor cells could be derived from this 3D induction system. Colony-forming-unit (CFU) assay showed that iPSC-derived CD34(+) cells formed all types of hematopoietic colonies including CFU-GEMM. TAL-1 and MIXL1, critical transcription factors associated with hematopoietic development, were expressed during the differentiation process. Furthermore, iPSC-derived hematopoietic cells gave rise to both lymphoid and myeloid lineages in the recipient NOD/SCID mice after transplantation. Conclusions: Our study underscores the importance of a synthetic 3D niche system for the derivation of transplantable hematopoietic cells from human iPSCs in vitro thereby establishing a foundation towards utilization of human iPSC-derived HSCs for transplantation therapies in the clinic.

引用

页码：1 / 16

页数：16

共 60 条

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