Effect of divalent cations on permeabilizer-induced lysozyme lysis of Pseudomonas aeruginosa

被引：22

作者：

Ayres, HM ^{[1
]}

Furr, JR ^{[1
]}

Russell, AD ^{[1
]}

机构：

[1] Univ Wales Coll Cardiff, Welsh Sch Pharm, Cardiff CF1 3XF, S Glam, Wales

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1998年 / 27卷 / 06期

关键词：

D O I：

10.1046/j.1472-765X.1998.00455.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The presence of divalent (Mg2+) ions greatly reduced the lysis of Pseudomonas aeruginosa strain G48 in a system at pH 7.8 or 9.0 consisting of ethylenediamine tetraacetic acid (EDTA), lysozyme and tris. Similar reductions in lysis occurred when EDTA was replaced by nitrilotriacetic acid, sodium citrate or sodium polyphosphate. The effect depended on the cation concentration. Mg2+ ma!; replace cations removed from the outer membrane, or may effectively remove the permeabilizer from the system. The results suggest that the permeabilizing activity associated with these agents against this organism has a common basis in affecting the outer membrane.

引用

页码：372 / 374

页数：3

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