Two-photon time-resolved confocal microscopy using a digital micromirror device

被引：0
|
作者：
Schellenberg, M. [1 ]
Peev, E. [1 ]
Kloster, M. [1 ]
Napier, J. [1 ]
Neu, W. [1 ]
机构：
[1] Univ Appl Sci Emden Leer, Dept Nat Sci, D-26723 Emden, Germany
来源：
EMERGING DIGITAL MICROMIRROR DEVICE BASED SYSTEMS AND APPLICATIONS III | 2011年 / 7932卷
关键词：
two-photon; DMD; confocal microscopy; fluorescence microscopy; 4D-microscopy; femtosecond-laser; SECTIONS;
D O I：
10.1117/12.874681
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
By two-photon time-resolved confocal 4D-microscopy it is possible to image fluorescent objects at a high spatial and temporal resolution. The usage of femtosecond-laser light creates a two photon effect and therefore reduces bleaching of the fluorophore. With this technique 4D-visualization of dynamic processes in living cells is possible.
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页数：11
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