Reduced faecal shedding of Escherichia coli O157:H7 in cattle following systemic vaccination with γ-intimin C280 and EspB proteins

被引：43

作者：

Vilte, Daniel A. ^{[1
]}

Larzabal, Mariano ^{[2
]}

Garbaccio, Sergio ^{[1
]}

Gammella, Mariela ^{[1
]}

Rabinovitz, Bettina C. ^{[1
]}

Elizondo, Ana M. ^{[1
]}

Cantet, Rodolfo J. C. ^{[3
]}

Delgado, Fernando ^{[1
]}

Meikle, Virginia ^{[2
]}

Cataldi, Angel ^{[2
]}

Mercado, Elsa C. ^{[1
]}

机构：

[1] INTA, Inst Patobiol, RA-1712 Castelar, Argentina

[2] INTA, Inst Biotecnol, RA-1712 Castelar, Argentina

[3] Univ Buenos Aires, Dept Anim Prod, Fac Agron, CONICET, RA-1417 Buenos Aires, DF, Argentina

来源：

VACCINE | 2011年 / 29卷 / 23期

关键词：

E. coli O157:H7; Intimin; EspB; Vaccine; III SECRETED PROTEINS; SHIGA TOXIN; EXPERIMENTAL-INFECTION; O157-H7; COLONIZATION; ANTIBODY-RESPONSES; IMMUNE-RESPONSES; HUMAN COLOSTRUM; PREVALENCE; ADHERENCE; O157H7;

D O I：

10.1016/j.vaccine.2011.03.079

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is the most prevalent EHEC serotype that has been recovered from patients with haemolytic uremic syndrome (HUS) worldwide. Vaccination of cattle, the main reservoir of EHEC O157:H7, could be a logical strategy to fight infection in humans. This study evaluated a vaccine based on the carboxyl-terminal fragment of 280 amino acids of gamma-intimin (gamma-intimin C-280) and EspB, two key colonization factors of E. coli O157:H7. Intramuscular immunization elicited significantly high levels of serum IgG antibodies against both proteins. Antigen-specific IgA and IgG were also induced in saliva, but only the IgA response was significant. Following experimental challenge with E. coli O157:H7, a significant reduction in bacterial shedding was observed in vaccinated calves, compared to control group. These promising results suggest that systemic immunization of cattle with intimin and EspB could be a feasible strategy to reduce EHEC O157:H7 faecal shedding in cattle. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：3962 / 3968

页数：7

共 63 条

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