Effect of pretreatment and enzymatic hydrolysis of wheat straw on cell wall composition, hydrophobicity and cellulase adsorption

被引：81

作者：

Heiss-Blanquet, Senta ^{[1
]}

Zheng, Dan ^{[2
]}

Lopes Ferreira, Nicolas ^{[1
]}

Lapierre, Catherine ^{[2
]}

Baumberger, Stephanie ^{[2
]}

机构：

[1] IFP Energies Nouvelles, F-92852 Rueil Malmaison, France

[2] AgroParisTech INRA, Inst Jean Pierre Bourgin, UMR 1318, F-78026 Versailles, France

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 10期

关键词：

Lignin; Hydrophobicity; Cellulases; Adsorption; Saccharification; TRICHODERMA-REESEI; STEAM EXPLOSION; LIGNOCELLULOSIC BIOMASS; CELLOBIOHYDROLASE-I; MICROCRYSTALLINE CELLULOSE; ENDOGLUCANASE II; RICE STRAW; LIGNIN; DIGESTIBILITY; ACCESSIBILITY;

D O I：

10.1016/j.biortech.2011.03.011

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The present study aimed to determine the impact of cell wall composition and lignin content on enzyme adsorption and degradability. Thioacidolysis analysis of residual lignins in wheat straw after steam-explosion or organosolv pretreatment revealed an increase in lignin condensation degree of 27% and 33%, respectively. Surface hydrophobicity assessed through wettability tests decreased after the pretreatments (contact angle decrease of 20-50%), but increased with enzymatic conversion (30% maximum contact angle increase) and correlatively to lignin content. Adsorption of the three major cellulases Cel7A, Cel6A and Cel7B from Trichoderma reesei decreased with increasing hydrolysis time, down to 7%, 31% and 70% on the sample with the highest lignin content, respectively. The fraction of unspecifically bound enzymes was dependent both on the enzyme and the lignin content. Adsorption and specific activity were shown to be inversely proportional to lignin content and hydrophobicity, suggesting that lignin is one of the factors restricting enzymatic hydrolysis. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：5938 / 5946

页数：9

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