Immunogenicity of a novel tetravalent vaccine formulation with four recombinant lipidated dengue envelope protein domain IIIs in mice

被引：30

作者：

Chiang, Chen-Yi ^{[1
]}

Pan, Chien-Hsiung ^{[1
,2
]}

Chen, Mei-Yu ^{[1
]}

Hsieh, Chun-Hsiang ^{[1
]}

Tsai, Jy-Ping ^{[1
]}

Liu, Hsueh-Hung ^{[1
]}

Liu, Shih-Jen ^{[1
,2
]}

Chong, Pele ^{[1
,2
]}

Leng, Chih-Hsiang ^{[1
,2
]}

Chen, Hsin-Wei ^{[1
,2
]}

机构：

[1] Natl Hlth Res Inst, Natl Inst Infect Dis & Vaccinol, 35 Keyan Rd, Miaoli 350, Taiwan

[2] China Med Univ, Grad Inst Immunol, Taichung, Taiwan

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

CD8(+) T-CELL; NEUTRALIZING ANTIBODIES; EPIDEMIOLOGIC TRENDS; FUSION PROTEIN; GLOBAL DISTRIBUTION; PROTECTIVE ROLE; E-GLYCOPROTEIN; DNA VACCINE; VIRUS; RESPONSES;

D O I：

10.1038/srep30648

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We developed a novel platform to express high levels of recombinant lipoproteins with intrinsic adjuvant properties. Based on this technology, our group developed recombinant lipidated dengue envelope protein domain IIIs as vaccine candidates against dengue virus. This work aims to evaluate the immune responses in mice to the tetravalent formulation. We demonstrate that 4 serotypes of recombinant lipidated dengue envelope protein domain III induced both humoral and cellular immunity against all 4 serotypes of dengue virus on the mixture that formed the tetravalent formulation. Importantly, the immune responses induced by the tetravalent formulation in the absence of the exogenous adjuvant were functional in clearing the 4 serotypes of dengue virus in vivo. We affirm that the tetravalent formulation of recombinant lipidated dengue envelope protein domain III is a potential vaccine candidate against dengue virus and suggest further detailed studies of this formulation in nonhuman primates.

引用

页数：10

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