Ss-Bi1 encodes a putative BAX inhibitor-1 protein that is required for full virulence of Sclerotinia sclerotiorum

被引：30

作者：

Yu, Yang ^{[1
]}

Xiao, Jifen ^{[1
]}

Yang, Yuheng ^{[1
]}

Bi, Chaowei ^{[1
]}

Qing, Ling ^{[1
]}

Tan, Wanzhong ^{[1
]}

机构：

[1] Southwest Univ, Coll Plant Protect, Chongqing 400715, Peoples R China

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2015年 / 90卷

基金：

中国国家自然科学基金;

关键词：

Sclerotinia sclerotiorum; Bax inhibitor-1; Virulence; Stress response; PROGRAMMED CELL-DEATH; OXALIC-ACID; ENDO-POLYGALACTURONASES; SUPEROXIDE-DISMUTASE; GENE; APOPTOSIS; IDENTIFICATION; PATHOGENICITY; SUPPRESSOR; INFECTION;

D O I：

10.1016/j.pmpp.2015.04.005

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Sclerotinia sclerotiorum is a destructive necrotrophic plant pathogen with global distribution. Although S. sclerotiorum has been studied extensively, substantial research on aspects of the pathogen's ability to cause disease is still needed. Bax inhibitor-1 protein functions as a suppressor of programmed cell death and is involved in the response to biotic and abiotic stress in animals, plants and yeast. In this study, we functionally characterized a putative Bax inhibitor-1 protein, Ss-Bi1, from S. sclerotiorum. Ss-Bi1 is predicted to contain a BAX inhibitor-1-like super family domain and shows significant homology with many BAX inhibitor-1 proteins. High expression levels of Ss-Bi1 were observed in hyphae under various stresses. Targeted silencing of Ss-Bi1 resulted in reduced virulence in host plants. Ss-Bi1 gene-silenced strains were more sensitive to heat stress and ER stress than the wild-type strain. The results suggest that Ss-Bi1 encodes a putative BAX inhibitor-1 protein that is required for full virulence of S. sclerotiorum. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：115 / 122

页数：8

共 51 条

[31] CDD: a Conserved Domain Database for the functional annotation of proteins [J].

Marchler-Bauer, Aron ;

Lu, Shennan ;

Anderson, John B. ;

Chitsaz, Farideh ;

Derbyshire, Myra K. ;

DeWeese-Scott, Carol ;

Fong, Jessica H. ;

Geer, Lewis Y. ;

Geer, Renata C. ;

Gonzales, Noreen R. ;

Gwadz, Marc ;

Hurwitz, David I. ;

Jackson, John D. ;

Ke, Zhaoxi ;

Lanczycki, Christopher J. ;

Lu, Fu ;

Marchler, Gabriele H. ;

Mullokandov, Mikhail ;

Omelchenko, Marina V. ;

Robertson, Cynthia L. ;

Song, James S. ;

Thanki, Narmada ;

Yamashita, Roxanne A. ;

Zhang, Dachuan ;

Zhang, Naigong ;

Zheng, Chanjuan ;

Bryant, Stephen H. .

NUCLEIC ACIDS RESEARCH, 2011, 39 :D225-D229

[32] Purification of endo polygalacturonases from Sclerotinia sclerotiorum: Multiplicity of the complex enzyme system [J].

Martel, MB ;

Letoublon, R ;

Fevre, M .

CURRENT MICROBIOLOGY, 1996, 33 (04) :243-248

[33]

Poussereau N, 2001, FEMS MICROBIOL LETT, V194, P27, DOI 10.1111/j.1574-6968.2001.tb09441.x

[34] PRODUCTION OF CELL WALL-DEGRADING ENZYMES BY THE PHYTOPATHOGENIC FUNGUS SCLEROTINIA-SCLEROTIORUM [J].

RIOU, C ;

FREYSSINET, G ;

FEVRE, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (05) :1478-1484

[35] Bax Inhibitor 1 in apoptosis and disease [J].

Robinson, K. S. ;

Clements, A. ;

Williams, A. C. ;

Berger, C. N. ;

Frankel, G. .

ONCOGENE, 2011, 30 (21) :2391-2400

[36] The Sclerotinia sclerotiorum pac1 gene is required for sclerotial development and virulence [J].

Rollins, JA .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2003, 16 (09) :785-795

[37] PH signaling in Sclerotinia sclerotiorum:: Identification of a pacC/RIM1 Homolog [J].

Rollins, JA ;

Dickman, MB .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (01) :75-81

[38] Anti-Apoptotic Machinery Protects the Necrotrophic Fungus Botrytis cinerea from Host-Induced Apoptotic-Like Cell Death during Plant Infection [J].

Shlezinger, Neta ;

Minz, Anna ;

Gur, Yonatan ;

Hatam, Ido ;

Dagdas, Yasin F. ;

Talbot, Nicholas J. ;

Sharon, Amir .

PLOS PATHOGENS, 2011, 7 (08)

[39] MEMBRANE-PROTEIN STRUCTURE PREDICTION - HYDROPHOBICITY ANALYSIS AND THE POSITIVE-INSIDE RULE [J].

VONHEIJNE, G .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 225 (02) :487-494

[40] IDENTIFICATION OF A NOVEL CONSERVED HUMAN GENE, TEGT [J].

WALTER, L ;

MARYNEN, P ;

SZPIRER, J ;

LEVAN, G ;

GUNTHER, E .

GENOMICS, 1995, 28 (02) :301-304

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