Ss-Bi1 encodes a putative BAX inhibitor-1 protein that is required for full virulence of Sclerotinia sclerotiorum

被引：30
作者：
Yu, Yang [1 ]
Xiao, Jifen [1 ]
Yang, Yuheng [1 ]
Bi, Chaowei [1 ]
Qing, Ling [1 ]
Tan, Wanzhong [1 ]
机构：
[1] Southwest Univ, Coll Plant Protect, Chongqing 400715, Peoples R China
来源：
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2015年 / 90卷
基金：
中国国家自然科学基金;
关键词：
Sclerotinia sclerotiorum; Bax inhibitor-1; Virulence; Stress response; PROGRAMMED CELL-DEATH; OXALIC-ACID; ENDO-POLYGALACTURONASES; SUPEROXIDE-DISMUTASE; GENE; APOPTOSIS; IDENTIFICATION; PATHOGENICITY; SUPPRESSOR; INFECTION;
D O I：
10.1016/j.pmpp.2015.04.005
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Sclerotinia sclerotiorum is a destructive necrotrophic plant pathogen with global distribution. Although S. sclerotiorum has been studied extensively, substantial research on aspects of the pathogen's ability to cause disease is still needed. Bax inhibitor-1 protein functions as a suppressor of programmed cell death and is involved in the response to biotic and abiotic stress in animals, plants and yeast. In this study, we functionally characterized a putative Bax inhibitor-1 protein, Ss-Bi1, from S. sclerotiorum. Ss-Bi1 is predicted to contain a BAX inhibitor-1-like super family domain and shows significant homology with many BAX inhibitor-1 proteins. High expression levels of Ss-Bi1 were observed in hyphae under various stresses. Targeted silencing of Ss-Bi1 resulted in reduced virulence in host plants. Ss-Bi1 gene-silenced strains were more sensitive to heat stress and ER stress than the wild-type strain. The results suggest that Ss-Bi1 encodes a putative BAX inhibitor-1 protein that is required for full virulence of S. sclerotiorum. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：115 / 122
页数：8
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