Identifying orthogonal and similar reversed phase liquid chromatography stationary phases using the system selectivity cube and the hydrophobic subtraction model

被引：27

作者：

Johnson, Andrew R. ^{[2
]}

Johnson, Carrie M. ^{[3
]}

Stoll, Dwight R. ^{[3
]}

Vitha, Mark F. ^{[1
]}

机构：

[1] Drake Univ, Dept Chem, Des Moines, IA 50311 USA

[2] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

[3] Gustavus Adolphus Coll, Dept Chem, St Peter, MN 56082 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2012年 / 1249卷

关键词：

System selectivity cube; Hydrophobic subtraction model; Column selectivity; Reversed phase; Orthogonal separation; Snyder-Dolan model; SOLVATION ENERGY RELATIONSHIPS; PRINCIPAL COMPONENT ANALYSIS; ALKYL-SILICA COLUMNS; FLUID CHROMATOGRAPHY; CLASSIFICATION; SEPARATION; RETENTION; PACKINGS; CYANOPROPYL; SCHEME;

D O I：

10.1016/j.chroma.2012.05.049

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have compared over 500 RPLC columns characterized by the hydrophobic subtraction model using the system selectivity cube (SSC). We have shown numerous differences in column selectivity even among columns in the same class (e.g., alkyl-silica, cyan, or embedded polar groups). We also illustrate the utility of our method for selecting alternative columns with different selectivities for problematic separations and for selecting orthogonal columns for use in two-dimensional separations. The system selectivity cube offers a visual way to easily compare many columns simultaneously and select those columns offering the desired selectivity. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：62 / 82

页数：21

共 54 条

[1] Human skin permeation and partition: General linear free-energy relationship analyses [J].