Quantitative phase and refractive index measurements with point-source digital in-line holographic microscopy

被引:54
作者
Jericho, M. H. [1 ]
Kreuzer, H. J. [1 ]
Kanka, M. [2 ]
Riesenberg, R. [2 ]
机构
[1] Dalhousie Univ, Dept Phys & Atmospher Sci, Halifax, NS B3H 3J5, Canada
[2] Inst Photon Technol, D-07747 Jena, Germany
基金
加拿大自然科学与工程研究理事会;
关键词
RECONSTRUCTION;
D O I
10.1364/AO.51.001503
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Point-source digital in-line holographic microscopy with numerical reconstruction is ideally suited for quantitative phase measurements to determine optical path lengths and to extract changes in refractive index within accuracy close to 0.001 on the submicrometer length scale. This is demonstrated with simulated holograms and with detailed measurements on a number of different micrometer-sized samples such as suspended drops, optical fibers, as well as organisms of biological interest such as E. coli bacteria, HeLa cells, and fibroblast cells. (C) 2012 Optical Society of America
引用
收藏
页码:1503 / 1515
页数:13
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