Graphene oxide-based selection and identification of ofloxacin-specific single-stranded DNA aptamers

被引：16

作者：

Zhang, Yuhong ^{[1
]}

You, Yuanding ^{[1
]}

Xia, Ziwei ^{[1
]}

Han, Xuyan ^{[1
]}

Tian, Yaping ^{[1
]}

Zhou, Nandi ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China

来源：

RSC ADVANCES | 2016年 / 6卷 / 101期

基金：

中国国家自然科学基金;

关键词：

COLORIMETRIC DETECTION; ANTIBIOTICS; QUINOLONE; DESIGN; SELEX;

D O I：

10.1039/c6ra18430h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Graphene oxide (GO) was introduced in the process of systematic evolution of ligands by exponential enrichment (SELEX) to screen ofloxacin-specific single-stranded DNA aptamers. Four sequences with high homology, ap1, ap3, ap4 and ap5 were picked out. Their secondary structures were simulated, and the dissociation constants (K-d) were measured, which were 251.3, 130.1, 159.1 and 304.4 nM, respectively. The specificity of the aptamers was also evaluated.

引用

页码：99540 / 99545

页数：6

共 50 条

[41] Development of human CBF1-targeting single-stranded DNA aptamers as angiogenic inhibitors
Kagajo, Mari
Hatta, Yoshiko
Maekawa, Masashi
Ogawa, Atsushi
Ishimae, Minenori
Egushi, Mariko
Higashiyama, Shigeki
CANCER SCIENCE, 2021, 112 : 887 - 887
[42] ACTION OF SINGLE-STRANDED DNA SPECIFIC NUCLEASE S1 ON DOUBLE-STRANDED DNA
GODSON, GN
BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 308 (01) : 59 - 67
[43] In vitro identification of single-stranded DNA aptamers targeting human IL-23 using the protein-SELEX strategy
Rezaee, Mohammad Ali
Shobeiri, Saeideh Sadat
Moghadam, Malihe
Mashayekhi, Kazem
Sankian, Mojtaba
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2024, 247
[44] Detection of single-stranded DNA using the Dirac voltage change of graphene-based FETs
Yi, Jong Chang
Kim, Young June
Kim, Yi Young
Lee, Seok
Woo, Deok Ha
Kim, Chulki
PROCEEDINGS OF THE 2019 IEEE REGION 10 CONFERENCE (TENCON 2019): TECHNOLOGY, KNOWLEDGE, AND SOCIETY, 2019, : 2051 - 2053
[45] ENDONUCLEASE ACTIVITY OF VENOM PHOSPHODIESTERASE SPECIFIC FOR SINGLE-STRANDED AND SUPERHELICAL DNA
PRITCHARD, AE
KOWALSKI, D
LASKOWSKI, M
JOURNAL OF BIOLOGICAL CHEMISTRY, 1977, 252 (23) : 8652 - 8659
[46] Sequence-specific binding of Ku autoantigen to single-stranded DNA
Torrance, H
Giffin, W
Rodda, DJ
Pope, L
Haché, RJG
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (33) : 20810 - 20819
[47] INTRACELLULAR ENDONUCLEASE OF BACILLUS-SUBTILIS SPECIFIC FOR SINGLE-STRANDED DNA
CIARROCCHI, G
FORTUNATO, A
COBIANCHI, F
FALASCHI, A
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 61 (02): : 487 - 492
[48] ISOLATION OF NEUROSPORA-CRASSA ENDONUCLEASE SPECIFIC FOR SINGLE-STRANDED DNA
RABIN, EZ
FRASER, MJ
CANADIAN JOURNAL OF BIOCHEMISTRY, 1970, 48 (03): : 389 - &
[49] Exponential amplification of DNA with very low background using graphene oxide and single-stranded binding protein to suppress non-specific amplification
Wang, Jianping
Zou, Bingjie
Rui, Jianzhong
Song, Qinxin
Kajiyama, Tomoharu
Kambara, Hideki
Zhou, Guohua
MICROCHIMICA ACTA, 2015, 182 (5-6) : 1095 - 1101
[50] Identification of Bacteria by Conjugated Oligoelectrolyte/Single-Stranded DNA Electrostatic Complexes
Duarte, Aidee
Chworos, Arkadiusz
Flagan, Suvi F.
Hanrahan, Grady
Bazan, Guillermo C.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (36) : 12562 - 12564

← 1 2 3 4 5 →