Graphene oxide-based selection and identification of ofloxacin-specific single-stranded DNA aptamers

被引：15

作者：

Zhang, Yuhong ^{[1
]}

You, Yuanding ^{[1
]}

Xia, Ziwei ^{[1
]}

Han, Xuyan ^{[1
]}

Tian, Yaping ^{[1
]}

Zhou, Nandi ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China

来源：

RSC ADVANCES | 2016年 / 6卷 / 101期

基金：

中国国家自然科学基金;

关键词：

COLORIMETRIC DETECTION; ANTIBIOTICS; QUINOLONE; DESIGN; SELEX;

D O I：

10.1039/c6ra18430h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Graphene oxide (GO) was introduced in the process of systematic evolution of ligands by exponential enrichment (SELEX) to screen ofloxacin-specific single-stranded DNA aptamers. Four sequences with high homology, ap1, ap3, ap4 and ap5 were picked out. Their secondary structures were simulated, and the dissociation constants (K-d) were measured, which were 251.3, 130.1, 159.1 and 304.4 nM, respectively. The specificity of the aptamers was also evaluated.

引用

页码：99540 / 99545

页数：6

共 41 条

[31] Selection of DNA aptamers for tramadol through the systematic evolution of ligands by exponential enrichment method for fabrication of a sensitive fluorescent aptasensor based on graphene oxide
Hedayati, Narges
Taghdisi, Seyed Mohammad
Yazdian-Robati, Rezvan
Mansouri, Atena
Abnous, Khalil
Mohajeri, Seyed Ahmad
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2021, 259
[32] Selection of Specific DNA Aptamers for Hetero-Sandwich-Based Colorimetric Determination of Campylobacter jejuni in Food
Chen, Wei
Teng, Jun
Yao, Li
Xu, Jianguo
Liu, Guodong
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2020, 68 (31) : 8455 - 8461
[33] Dual color fluorescence quantitative detection of specific single-stranded DNA with molecular beacons and nucleic acid dye SYBR Green I
Xiang, Dong-Shan
Zhou, Guo-Hua
Luo, Ming
Ji, Xing-Hu
He, Zhi-Ke
ANALYST, 2012, 137 (16) : 3787 - 3793
[34] Rapid Identification of Cell-Specific, Internalizing RNA Aptamers with Bioinformatics Analyses of a Cell-Based Aptamer Selection
Thiel, William H.
Bair, Thomas
Peek, Andrew S.
Liu, Xiuying
Dassie, Justin
Stockdale, Katie R.
Behlke, Mark A.
Miller, Francis J., Jr.
Giangrande, Paloma H.
PLOS ONE, 2012, 7 (09):
[35] Novel aptasensor for the ultrasensitive detection of kanamycin based on grapheneoxide quantum-dot-linked single-stranded DNA-binding protein
He, Yanhua
Wen, Xiaoye
Zhang, Bingyan
Fan, Zhefeng
SENSORS AND ACTUATORS B-CHEMICAL, 2018, 265 : 20 - 26
[36] A fluorescent aptasensor for analysis of adenosine triphosphate based on aptamer-magnetic nanoparticles and its single-stranded complementary DNA labeled carbon dots
Saberi, Zeinab
Rezaei, Behzad
Khayamian, Taghi
LUMINESCENCE, 2018, 33 (04) : 640 - 646
[37] Fluorescence Resonance Energy Transfer-Based Photonic Circuits Using Single-Stranded Tile Self-Assembly and DNA Strand Displacement
Zhang, Xuncai
Ying, Niu
Shen, Chaonan
Cui, Guangzhao
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2017, 17 (02) : 1053 - 1060
[38] Selection of HBsAg-Specific DNA Aptamers Based on Carboxylated Magnetic Nanoparticles and Their Application in the Rapid and Simple Detection of Hepatitis B Virus Infection
Xi, Zhijiang
Huang, Rongrong
Li, Zhiyang
He, Nongyue
Wang, Ting
Su, Enben
Deng, Yan
ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (21) : 11215 - 11223
[39] Selection of specific aptamer against enrofloxacin and fabrication of graphene oxide based label-free fluorescent assay
Dolati, Somayeh
Ramezani, Mohammad
Nabavinia, Maryam Sadat
Soheili, Vahid
Abnous, Khalil
Taghdisi, Seyed Mohammad
ANALYTICAL BIOCHEMISTRY, 2018, 549 : 124 - 129
[40] DNA engineered copper oxide-based nanocomposites with multiple enzyme-like activities for specific detection of mercury species in environmental and biological samples
Lien, Chia-Wen
Yu, Po-Hsiung
Chang, Huan-Tsung
Hsu, Pang-Hung
Wu, Tsunghsueh
Lin, Yang-Wei
Huang, Chih-Ching
Lai, Jui-Yang
ANALYTICA CHIMICA ACTA, 2019, 1084 : 106 - 115

← 1 2 3 4 5 →