Graphene oxide-based selection and identification of ofloxacin-specific single-stranded DNA aptamers

被引：16

作者：

Zhang, Yuhong ^{[1
]}

You, Yuanding ^{[1
]}

Xia, Ziwei ^{[1
]}

Han, Xuyan ^{[1
]}

Tian, Yaping ^{[1
]}

Zhou, Nandi ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China

来源：

RSC ADVANCES | 2016年 / 6卷 / 101期

基金：

中国国家自然科学基金;

关键词：

COLORIMETRIC DETECTION; ANTIBIOTICS; QUINOLONE; DESIGN; SELEX;

D O I：

10.1039/c6ra18430h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Graphene oxide (GO) was introduced in the process of systematic evolution of ligands by exponential enrichment (SELEX) to screen ofloxacin-specific single-stranded DNA aptamers. Four sequences with high homology, ap1, ap3, ap4 and ap5 were picked out. Their secondary structures were simulated, and the dissociation constants (K-d) were measured, which were 251.3, 130.1, 159.1 and 304.4 nM, respectively. The specificity of the aptamers was also evaluated.

引用

页码：99540 / 99545

页数：6

共 50 条

[31] Fast and simple method for screening of single-stranded DNA breaking photosensitizers using graphene oxide
Joong Hyun Kim
Hyun Jin Kim
Nano Convergence, 5
[32] Fast and simple method for screening of single-stranded DNA breaking photosensitizers using graphene oxide
Kim, Joong Hyun
Kim, Hyun Jin
NANO CONVERGENCE, 2018, 5
[33] Selection and Characterization of Single Stranded DNA Aptamers for the Hormone Abscisic Acid
Grozio, Alessia
Gonzalez, Victor M.
Millo, Enrico
Sturla, Laura
Vigliarolo, Tiziana
Bagnasco, Luca
Guida, Lucrezia
D'Arrigo, Cristina
De Flora, Antonio
Salis, Annalisa
Martin, Elena M.
Bellotti, Marta
Zocchi, Elena
NUCLEIC ACID THERAPEUTICS, 2013, 23 (05) : 322 - 331
[34] SEQUENCE SPECIFIC METHYLATION OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA BY METHYLNITROSOUREA
WURDEMAN, RW
GOLD, B
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1992, 204 : 154 - AGFD
[35] Characterization of pharmacological properties of isolated single-stranded DNA aptamers against angiotensin II
Heiat, Mohammad
Ranjbar, Reza
Fasihi-Ramandi, Mandi
Latifi, Ali Mohammad
Rasaee, Mohammad Javad
MOLECULAR AND CELLULAR PROBES, 2016, 30 (04) : 238 - 245
[36] Selection of DNA aptamers to Streptococcus pneumonia and fabrication of graphene oxide based fluorescent assay
Bayrac, Abdullah Tahir
Donmez, Sultan Ilayda
ANALYTICAL BIOCHEMISTRY, 2018, 556 : 91 - 98
[37] Selection and Application of DNA Aptamers Against Sulfaquinoxaline Assisted by Graphene Oxide–Based SELEX
Haixing Shi
Qiming Kou
Ping Wu
Qi Sun
Juan Wu
Tao Le
Food Analytical Methods, 2021, 14 : 250 - 259
[38] Thrombin inhibitors based on single- stranded DNA aptamers
Gribkova, Irina V.
Spiridonova, Vera A.
Gorbatenko, Alexander S.
Denisov, Stepan S.
Ataullakhanov, Fazoil I.
Sinauridze, Elena I.
BLOOD COAGULATION & FIBRINOLYSIS, 2014, 25 (01) : 39 - 45
[39] Graphene Oxide-Based Nanostructured DNA Sensor
Balaji, Aditya
Yang, Songlin
Wang, Jeslyn
Zhang, Jin
BIOSENSORS-BASEL, 2019, 9 (02):
[40] SEQUENCE-SPECIFIC METHYLATION OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA BY METHYLNITROSOUREA
WURDEMAN, RW
GOLD, B
NITROSAMINES AND RELATED N-NITROSO COMPOUNDS: CHEMISTRY AND BIOCHEMISTRY, 1994, 553 : 346 - 350

← 1 2 3 4 5 →