Graphene oxide-based selection and identification of ofloxacin-specific single-stranded DNA aptamers

被引：15

作者：

Zhang, Yuhong ^{[1
]}

You, Yuanding ^{[1
]}

Xia, Ziwei ^{[1
]}

Han, Xuyan ^{[1
]}

Tian, Yaping ^{[1
]}

Zhou, Nandi ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China

来源：

RSC ADVANCES | 2016年 / 6卷 / 101期

基金：

中国国家自然科学基金;

关键词：

COLORIMETRIC DETECTION; ANTIBIOTICS; QUINOLONE; DESIGN; SELEX;

D O I：

10.1039/c6ra18430h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Graphene oxide (GO) was introduced in the process of systematic evolution of ligands by exponential enrichment (SELEX) to screen ofloxacin-specific single-stranded DNA aptamers. Four sequences with high homology, ap1, ap3, ap4 and ap5 were picked out. Their secondary structures were simulated, and the dissociation constants (K-d) were measured, which were 251.3, 130.1, 159.1 and 304.4 nM, respectively. The specificity of the aptamers was also evaluated.

引用

页码：99540 / 99545

页数：6

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