Graphene oxide-based selection and identification of ofloxacin-specific single-stranded DNA aptamers

被引：16

作者：

Zhang, Yuhong ^{[1
]}

You, Yuanding ^{[1
]}

Xia, Ziwei ^{[1
]}

Han, Xuyan ^{[1
]}

Tian, Yaping ^{[1
]}

Zhou, Nandi ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China

来源：

RSC ADVANCES | 2016年 / 6卷 / 101期

基金：

中国国家自然科学基金;

关键词：

COLORIMETRIC DETECTION; ANTIBIOTICS; QUINOLONE; DESIGN; SELEX;

D O I：

10.1039/c6ra18430h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Graphene oxide (GO) was introduced in the process of systematic evolution of ligands by exponential enrichment (SELEX) to screen ofloxacin-specific single-stranded DNA aptamers. Four sequences with high homology, ap1, ap3, ap4 and ap5 were picked out. Their secondary structures were simulated, and the dissociation constants (K-d) were measured, which were 251.3, 130.1, 159.1 and 304.4 nM, respectively. The specificity of the aptamers was also evaluated.

引用

页码：99540 / 99545

页数：6

共 50 条

[1] Selection and identification of streptomycin-specific single-stranded DNA aptamers and the application in the detection of streptomycin in honey
Zhou, Nandi
Wang, Jingyuan
Zhang, Juan
Li, Can
Tian, Yaping
Wang, Joseph
TALANTA, 2013, 108 : 109 - 116
[2] Single-stranded DNA aptamers specific for antibiotics tetracyclines
Niazi, Javed H.
Lee, Su Jin
Gu, Man Bock
BIOORGANIC & MEDICINAL CHEMISTRY, 2008, 16 (15) : 7245 - 7253
[3] Development of Single-Stranded DNA Aptamers for Specific Bisphenol A Detection
Jo, Minjoung
Ahn, Ji-Young
Lee, Joohyung
Lee, Seram
Hong, Sun Woo
Yoo, Jae-Wook
Kang, Jeehye
Dua, Pooja
Lee, Dong-ki
Hong, Seunghun
Kim, Soyoun
OLIGONUCLEOTIDES, 2011, 21 (02) : 85 - 91
[4] Selection and Application of DNA Aptamers Against Sulfaquinoxaline Assisted by Graphene Oxide-Based SELEX
Shi, Haixing
Kou, Qiming
Wu, Ping
Sun, Qi
Wu, Juan
Le, Tao
FOOD ANALYTICAL METHODS, 2021, 14 (02) : 250 - 259
[5] Selection and characterization of single-stranded DNA aptamers against interleukin-5
Jamalvandi, Mina
Khanahmad, Hossein
Irani, Shiva
Bastaminezhad, Sayad
RESEARCH IN PHARMACEUTICAL SCIENCES, 2019, 14 (06) : 515 - 523
[6] Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications
Hong, Ka Lok
Sooter, Letha J.
BIOMED RESEARCH INTERNATIONAL, 2015, 2015
[7] Functionalization of graphene with single-stranded DNA
Sim, Yumin
Seong, Maeng-Je
JOURNAL OF THE KOREAN PHYSICAL SOCIETY, 2015, 67 (11) : 1952 - 1956
[8] Functionalization of graphene with single-stranded DNA
Yumin Sim
Maeng-Je Seong
Journal of the Korean Physical Society, 2015, 67 : 1952 - 1956
[9] In vitro selection of single-stranded DNA aptamers that bind human pro-urokinase
Skrypina, NA
Savochkina, LP
Beabealashvilli, RS
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 2004, 23 (6-7): : 891 - 893
[10] Rapid selection of single-stranded DNA aptamers binding Staphylococcus epidermidis in platelet concentrates
Kaur, Simran J.
Gilman, Vladimir
Duong, Minh
Asher, David M.
Gregori, Luisa
BIOTECHNIQUES, 2018, 65 (06) : 331 - 338

← 1 2 3 4 5 →