Domain versatility in plant AB-toxins:: Evidence for a local, pH-dependent rearrangement in the 2γ lectin site of the mistletoe lectin by applying ligand derivatives and modelling

被引：24

作者：

Jimenez, Marta ^{[1
]}

Andre, Sabine ^{[2
]}

Barillari, Caterina ^{[3
]}

Romero, Antonio ^{[4
]}

Rognan, Didier ^{[3
]}

Gabius, Hans-Joachim ^{[2
]}

Solis, Dolores ^{[1
]}

机构：

[1] CSIC, Inst Quim Fis Rocasolano, E-28006 Madrid, Spain

[2] Univ Munich, Tierarztliche Fak, Inst Physiol Chem, D-80539 Munich, Germany

[3] Univ Louis Pasteur Strasbourg 1, CNRS, UMR 7175, Lab Bioinformat Medicament, F-67400 Illkirch Graffenstaden, France

[4] CSIC, Ctr Invest Biol, E-28040 Madrid, Spain

来源：

FEBS LETTERS | 2008年 / 582卷 / 15期

关键词：

agglutinin; lectin; mistletoe; ribosome-inactivating protein; ricin;

D O I：

10.1016/j.febslet.2008.05.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mistletoe lectin is a potent biohazard. Lectin activity in the toxic dimer primarily originates from the 2 gamma-subdomain (Tyr-site) of the B-subunit. Crystallographic information on lectin-sugar complexes is available only at acidic pH, where lectin activity is low. Thus, we mapped ligand-binding properties including comparison to ricin's Tyr-site at neutral pH. Using these results and molecular dynamics simulations, a local conformational change was rendered likely. The obtained structural information is valuable for the design of potent inhibitors. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2309 / 2312

页数：4

共 27 条

[11] Fast, efficient generation of high-quality atomic charges. AM1-BCC model: II. Parameterization and validation
Jakalian, A
Jack, DB
Bayly, CI
[J]. JOURNAL OF COMPUTATIONAL CHEMISTRY, 2002, 23 (16) : 1623 - 1641
[12] Calibration of colorimetric protein assays for quantitation of plant AB toxins
Jiménez, M
André, S
Gabius, HJ
Solís, D
[J]. ANALYTICAL BIOCHEMISTRY, 2000, 284 (02) : 418 - 420
[13] Monomer/dimer equilibrium of the AB-type lectin from mistletoe enables combination of toxin/agglutinin activities in one protein:: analysis of native and citraconylated proteins by ultracentrifugation/gel filtration and cell biological consequences of dimer destabilization
Jiménez, M
Sáiz, JL
André, S
Gabius, HJ
Solís, D
[J]. GLYCOBIOLOGY, 2005, 15 (12) : 1386 - 1395
[14] AB-type lectin (toxin/agglutinin) from mistletoe:: differences in affinity of the two galactoside-binding Trp/Tyr-sites and regulation of their functionality by monomer/dimer equilibrium
Jimenez, Marta
Andre, Sabine
Siebert, Hans-C.
Gabius, Hans-J.
Solis, Dolores
[J]. GLYCOBIOLOGY, 2006, 16 (10) : 926 - 937
[15] LEE RT, 1992, J BIOL CHEM, V267, P23722
[16] Mistletoe lectin I in complex with galactose and lactose reveals distinct sugar-binding properties
Mikeska, R
Wacker, R
Arni, R
Singh, TP
Mikhailov, A
Gabdoulkhakov, A
Voelter, W
Betzel, C
[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2005, 61 : 17 - 25
[17] Differences in endocytosis and intracellular sorting of ricin and viscumin in 3T3 cells
Moisenovich, M
Tonevitsky, A
Agapov, I
Niwa, H
Schewe, H
Bereiter-Hahn, J
[J]. EUROPEAN JOURNAL OF CELL BIOLOGY, 2002, 81 (10) : 529 - 538
[18] Crystal structure at 3 Å of mistletoe lectin I, a dimeric type-II ribosome-inactivating protein, complexed with galactose
Niwa, H
Tonevitsky, AG
Agapov, II
Saward, S
Pfüller, U
Palmer, RA
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (13): : 2739 - 2749
[19] Plant lectins:: Occurrence, biochemistry, functions and applications
Rüdiger, H
Gabius, HJ
[J]. GLYCOCONJUGATE JOURNAL, 2001, 18 (08) : 589 - 613
[20] STRUCTURE OF RICIN B-CHAIN AT 2.5-A RESOLUTION
RUTENBER, E
ROBERTUS, JD
[J]. PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1991, 10 (03): : 260 - 269

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