Domain versatility in plant AB-toxins:: Evidence for a local, pH-dependent rearrangement in the 2γ lectin site of the mistletoe lectin by applying ligand derivatives and modelling

被引：24

作者：

Jimenez, Marta ^{[1
]}

Andre, Sabine ^{[2
]}

Barillari, Caterina ^{[3
]}

Romero, Antonio ^{[4
]}

Rognan, Didier ^{[3
]}

Gabius, Hans-Joachim ^{[2
]}

Solis, Dolores ^{[1
]}

机构：

[1] CSIC, Inst Quim Fis Rocasolano, E-28006 Madrid, Spain

[2] Univ Munich, Tierarztliche Fak, Inst Physiol Chem, D-80539 Munich, Germany

[3] Univ Louis Pasteur Strasbourg 1, CNRS, UMR 7175, Lab Bioinformat Medicament, F-67400 Illkirch Graffenstaden, France

[4] CSIC, Ctr Invest Biol, E-28040 Madrid, Spain

来源：

FEBS LETTERS | 2008年 / 582卷 / 15期

关键词：

agglutinin; lectin; mistletoe; ribosome-inactivating protein; ricin;

D O I：

10.1016/j.febslet.2008.05.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mistletoe lectin is a potent biohazard. Lectin activity in the toxic dimer primarily originates from the 2 gamma-subdomain (Tyr-site) of the B-subunit. Crystallographic information on lectin-sugar complexes is available only at acidic pH, where lectin activity is low. Thus, we mapped ligand-binding properties including comparison to ricin's Tyr-site at neutral pH. Using these results and molecular dynamics simulations, a local conformational change was rendered likely. The obtained structural information is valuable for the design of potent inhibitors. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2309 / 2312

页数：4

共 27 条

[1] NMR investigations of protein-carbohydrate interactions:: insights into the topology of the bound conformation of a lactose isomer and β-galactosyl xyloses to mistletoe lectin and galectin-1 [J].

Alonso-Plaza, JM ;

Canales, MA ;

Jiménez, M ;

Roldán, JL ;

García-Herrero, A ;

Iturrino, L ;

Asensio, JL ;

Cañada, FJ ;

Romero, A ;

Siebert, HC ;

André, S ;

Solís, D ;

Gabius, HJ ;

Jiménez-Barbero, J .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2001, 1568 (03) :225-236

[2] Glycosyldisulfides from dynamic combinatorial libraries as O-glycoside mimetics for plant and endogenous lectins:: Their reactivities in solid-phase and cell assays and conformational analysis by molecular dynamics simulations [J].

Andre, Sabine ;

Pei, Zhichao ;

Siebert, Hans-Christian ;

Ramstrom, Olof ;

Gabius, Hans-Joachim .

BIOORGANIC & MEDICINAL CHEMISTRY, 2006, 14 (18) :6314-6326

[3] Branching mode in complex-type triantennary N-glycans as regulatory element of their ligand properties [J].

André, Sabine ;

Kojima, Shuji ;

Gundel, Gislinde ;

Russwurm, Roland ;

Schratt, Xaver ;

Unverzagt, Carlo ;

Gabius, Hans-Joachim .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2006, 1760 (05) :768-782

[4]

BAENZIGER JU, 1979, J BIOL CHEM, V254, P9795

[5]

Case D.A., 2004, AMBER 8

[6] A SMOOTH PARTICLE MESH EWALD METHOD [J].

ESSMANN, U ;

PERERA, L ;

BERKOWITZ, ML ;

DARDEN, T ;

LEE, H ;

PEDERSEN, LG .

JOURNAL OF CHEMICAL PHYSICS, 1995, 103 (19) :8577-8593

[7]

GABIUS HJ, 1992, ANTICANCER RES, V12, P669

[8]

Galanina OE, 1997, J MOL RECOGNIT, V10, P139, DOI 10.1002/(SICI)1099-1352(199705/06)10:3<139::AID-JMR358>3.0.CO

[9]

2-R

[10] Binding diversity of the two binding sites of ricin B lectin [J].

Ganguly, Debabani ;

Mukhopadhyay, Chaitali .

BIOPOLYMERS, 2006, 83 (01) :83-94

← 1 2 3 →