Determination of Lead Concentration and Isotopic Ratios of Blood, Hair and Tissues in Rats by Inductively Coupled Plasma Mass Spectrometry

A method was developed to simultaneously determine lead concentration and lead isotopic ratios in blood, hair, and other organs of lead exposed rats by inductively coupled plasma mass spectrometry(ICP-MS). The measurement value of lead concentration in standard material is (278 4) mu g/L, that is in the range of certified value (283 +/- 18) mu g/L, and the RSDs of Ph concentration, ratio (204)pb/(206)pb, (207)pb/(206)pb and (208)pb/(206) Pb of in the determination of 10 mu g/L Pb standard solution were less than 0.7%, 0.3%, 0.15% and 0.15%, respectively. It was found that RSD of isotopic ratios is stable when Pb concentration is more than 8 mu g/L. The concentration calculated by sum of of all Pb isotopes is more accurate and reasonable than that only by Pb-208. The measured results of samples from rats tissues show that there is a selective accumulation of Pb in different tissues and organs, Pb concentration from high to low is in femur, kidney, hair, liver and blood. Blood is more suitable than hair for biomarker to reflect inner Pb accumulation.