Cu/Zn superoxide dismutase in yeast mitochondria - a general phenomenon

被引：22

作者：

Nedeva, TS ^{[1
]}

Petrova, VY ^{[1
]}

Zamfirova, DR ^{[1
]}

Stephanova, EV ^{[1
]}

Kujumdzieva, AV ^{[1
]}

机构：

[1] Univ Sofia, Fac Biol, Dept Gen & Ind Microbiol, Sofia 1164, Bulgaria

来源：

FEMS MICROBIOLOGY LETTERS | 2004年 / 230卷 / 01期

关键词：

fermentative/respiratory yeast; mitochondrion; superoxide dismutase;

D O I：

10.1016/S0378-1097(03)00855-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fermentative and respiratory yeast strains of genera Saccharomyces, Kluyveromyces, Pichia, Candida and Hansenula have been investigated for mitochondrial localization of Cu/Zn superoxide dismutase (SOD). Pure mitochondrial fractions were obtained and the specific activities of Cu/Zn and Mn SODs were measured in comparison with those in the corresponding cell-free extracts. The Cu/Zn SOD: Mn SOD ratio in mitochondria and crude extracts was calculated and was considered a specific characteristic of all tested strains. Electrophoretical visualization of SOD patterns provided evidence for possible migration of cytosolic Cu/Zn SOD to mitochondria. The characteristic Cu/Zn SOD profile in mitochondria of all tested strains suggested its ubiquity within the fermentative and respiratory yeasts. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：19 / 25

页数：7

共 19 条

[1]

Beauchamp C., 1971, ANAL BIOCHEM, V44, P276, DOI DOI 10.1016/0003-2697(71)90370-8

[2] SUCCINIC DEHYDROGENASE [J].

BONNER, WD .

METHODS IN ENZYMOLOGY, 1955, 1 :722-729

[3]

BRITTON L, 1978, J BACTERIOL, V134, P229, DOI 10.1128/JB.134.1.229-236.1978

[4]

CANCEDO C, 1989, YEASTS, V3, P205

[5] CITRATASE AND ISOCITRATASE [J].

DARON, HH ;

GUNSALUS, IC .

METHODS IN ENZYMOLOGY, 1962, 5 :622-633

[6] A RAPID MINIPREP METHOD FOR THE PREPARATION OF YEAST MITOCHONDRIAL-DNA [J].

DEFONTAINE, A ;

LECOCQ, FM ;

HALLET, JN .

NUCLEIC ACIDS RESEARCH, 1991, 19 (01) :185-185

[7]

FRIDOVICH I, 1986, ADV ENZYMOL RAMB, V58, P61

[8]

GELLER BL, 1984, METHOD ENZYMOL, V105, P105

[9] ENZYMES OF GLUCOSE CATABOLISM PATHWAYS IN COLLETOTRICHUM AND GLOEOSPORIUM [J].

GREENE, GL .

MYCOLOGIA, 1969, 61 (05) :902-&

[10] OXIDATION OF SPERMIDINE AND SPERMINE IN RAT-LIVER - PURIFICATION AND PROPERTIES OF POLYAMINE OXIDASE [J].

HOLTTA, E .

BIOCHEMISTRY, 1977, 16 (01) :91-100

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