Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities

被引:88
作者
Bacic, Goran [1 ]
Spasojevic, Ivan [2 ]
Secerov, Bojana [3 ]
Mojovic, Milos [1 ]
机构
[1] Fac Phys Chem, Belgrade 11000, Serbia
[2] Inst Multidisciplinary Res, Belgrade 11000, Serbia
[3] Vinca Inst Nucl Sci, Belgrade 11001, Serbia
关键词
EPR spectroscopy; spin-traps; DEPMPO; Fenton reaction; hydrogen radical;
D O I
10.1016/j.saa.2007.09.047
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an "ordinary" quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated hydrogen (H-center dot) atom. (C) 2007 Elsevier B.V. All rights reserved.
引用
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页码:1354 / 1366
页数:13
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