The effect of a submersion pretreatment on in vitro explant growth and shoot regeneration from hypocotyls of flax (Linum usitatissimum)

被引：18

作者：

Yildiz, M ^{[1
]}

Özgen, M ^{[1
]}

机构：

[1] Ankara Univ, Fac Agr, Dept Field Crops, TR-06110 Ankara, Turkey

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2004年 / 77卷 / 01期

关键词：

in vitro explant growth; in vitro shoot regeneration; Linum usitatissimum; tissue water content;

D O I：

10.1023/B:TICU.0000016493.03592.c3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study was carried out to determine the effect of temporary submersion of hypocotyl segments in water on in vitro explant growth and shoot regeneration on MS ( Murashige and Skoog, 1962) medium supplemented with 1 mg l(-1) BAP (6-benzylaminopurine) and 0.02 mg l(-1) NAA (naphthaleneacetic acid) in three flax cultivars. It was observed that water-treated hypocotyl explants gave rise to the highest values with respect to shoot regeneration percentage, shoot number per hypocotyl, shoot length and total shoot number per Petri dish, successful rooting and plantlet establishment. This procedure may be applicable for other species cultured in vitro.

引用

页码：111 / 115

页数：5

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