Two Cassava Basic Leucine Zipper (bZIP) Transcription Factors (MebZIP3 and MebZIP5) Confer Disease Resistance against Cassava Bacterial Blight

被引：51
作者：
Li, Xiaolin [1 ,2 ]
Fan, Shuhong [1 ,2 ]
Hu, Wei [3 ]
Liu, Guoyin [1 ,2 ]
Wei, Yunxie [1 ,2 ]
He, Chaozu [1 ,2 ]
Shi, Haitao [1 ,2 ]
机构：
[1] Hainan Univ, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou, Hainan, Peoples R China
[2] Hainan Univ, Coll Biol, Inst Trop Agr & Forestry, Haikou, Hainan, Peoples R China
[3] Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Haikou, Hainan, Peoples R China
来源：
FRONTIERS IN PLANT SCIENCE | 2017年 / 8卷
基金：
中国国家自然科学基金;
关键词：
basic leucine zipper (bZIP) transcription factor; cassava (Manihot esculenta); cassava bacterial blight; disease resistance; virus-induced gene silencing (VIGS); GENOME-WIDE CHARACTERIZATION; EXPRESSION ANALYSIS; ABIOTIC STRESSES; FACTOR FAMILY; GENE FAMILY; CELL-DEATH; ARABIDOPSIS; IDENTIFICATION; INFECTION; BIOSYNTHESIS;
D O I：
10.3389/fpls.2017.02110
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Basic domain-leucine zipper (bZIP) transcription factor, one type of conserved gene family, plays an important role in plant development and stress responses. Although 77 MebZIPs have been genome-wide identified in cassava, their in vivo roles remain unknown. In this study, we analyzed the expression pattern and the function of two MebZIPs (MebZIP3 and MebZIP5) in response to pathogen infection. Gene expression analysis indicated that MebZIP3 and MebZIP5 were commonly regulated by flg22, Xanthomonas axonopodis pv. manihotis (Xam), salicylic acid (SA), and hydrogen peroxide (H2O2). Subcellular localization analysis showed that MebZIP3 and MebZIP5 are specifically located in cell nucleus. Through overexpression in tobacco, we found that MebZIP3 and MebZIP5 conferred improved disease resistance against cassava bacterial blight, with more callose depositions. On the contrary, MebZIP3- and MebZIP5-silenced plants by virus-induced gene silencing (VIGS) showed disease sensitive phenotype, lower transcript levels of defense-related genes and less callose depositions. Taken together, this study highlights the positive role of MebZIP3 and MebZIP5 in disease resistance against cassava bacterial blight for further utilization in genetic improvement of cassava disease resistance.
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页数：11
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