Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/SS carrier DNA/PEG method

被引：452

作者：

Gietz, R. Daniel ^{[2
]}

Schiestl, Robert H. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Sch Publ Hlth, David Sch Med, Dept Pathol Environm & Hlth & Radiat Oncol, Los Angeles, CA 90095 USA

[2] Univ Manitoba, Dept Biochem & Med Genet, Winnipeg, MB R3E 0W3, Canada

来源：

NATURE PROTOCOLS | 2007年 / 2卷 / 01期

关键词：

D O I：

10.1038/nprot.2007.17

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe a protocol for the production of frozen competent yeast cells that can be transformed with high efficiency using the lithium acetate/single-stranded carrier DNA/PEG method. This protocol allows the production of highly competent yeast cells that can be frozen and used at a later date and is especially useful for laboratories using one or two strains repeatedly. The production of yeast cells for freezing takes only approximately 30 min, once the yeast culture has grown up. Transformation with frozen competent yeast cells will take approximately 30 min, depending on the heat shock used.

引用

页码：1 / 4

页数：4

共 12 条

[1]

Gietz D, 1998, METHOD MICROBIOL, V26, P53

[2]

Gietz R D, 2002, Methods Mol Biol, V185, P471

[3]

Gietz RD, 2007, NAT PROTOC, V2, P31, DOI 10.1038/nprot.2007.13

[4]

Gietz R Daniel, 2006, Methods Mol Biol, V313, P345