Evaluation of a DNA Microarray (Check-MDR CT102) for Rapid Detection of TEM, SHV, and CTX-M Extended-Spectrum β-Lactamases and of KPC, OXA-48, VIM, IMP, and NDM-1 Carbapenemases

被引：100
作者：
Naas, Thierry [1 ,2 ]
Cuzon, Gaelle [1 ,2 ]
Bogaerts, Pierre [3 ]
Glupczynski, Youri [3 ]
Nordmann, Patrice [1 ,2 ]
机构：
[1] Hop Bicetre, INSERM, U914, Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France
[2] AP HP, Fac Med Paris Sud, Paris, France
[3] Clin Univ UCL Mont Godinne, Bacteriol Lab, B-5530 Yvoir, Belgium
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 04期
关键词：
REAL-TIME PCR; GENES; ENTEROBACTERIACEAE; IDENTIFICATION; ASSAY; RESISTANCE; SYSTEM;
D O I：
10.1128/JCM.02607-10
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The Check-MDR CT102 microarray, aimed at identifying bacteria producing extended-spectrum beta-lactamase (ESBL) (SHV, TEM, and CTX-M) and carbapenemase (KPC, OXA-48, VIM, IMP, and NDM-1), was evaluated on a total of 144 Gram-negative strains expressing various beta-lactamases. The sensitivity and specificity were 100% for most tested genes, suggesting that this assay allows accurate identification of common ESBL and carbapenemase producers from bacterial cultures.
引用
收藏
页码：1608 / 1613
页数：6
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