Evaluation of a DNA Microarray (Check-MDR CT102) for Rapid Detection of TEM, SHV, and CTX-M Extended-Spectrum β-Lactamases and of KPC, OXA-48, VIM, IMP, and NDM-1 Carbapenemases

被引：101

作者：

Naas, Thierry ^{[1
,2
]}

Cuzon, Gaelle ^{[1
,2
]}

Bogaerts, Pierre ^{[3
]}

Glupczynski, Youri ^{[3
]}

Nordmann, Patrice ^{[1
,2
]}

机构：

[1] Hop Bicetre, INSERM, U914, Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France

[2] AP HP, Fac Med Paris Sud, Paris, France

[3] Clin Univ UCL Mont Godinne, Bacteriol Lab, B-5530 Yvoir, Belgium

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 04期

关键词：

REAL-TIME PCR; GENES; ENTEROBACTERIACEAE; IDENTIFICATION; ASSAY; RESISTANCE; SYSTEM;

D O I：

10.1128/JCM.02607-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Check-MDR CT102 microarray, aimed at identifying bacteria producing extended-spectrum beta-lactamase (ESBL) (SHV, TEM, and CTX-M) and carbapenemase (KPC, OXA-48, VIM, IMP, and NDM-1), was evaluated on a total of 144 Gram-negative strains expressing various beta-lactamases. The sensitivity and specificity were 100% for most tested genes, suggesting that this assay allows accurate identification of common ESBL and carbapenemase producers from bacterial cultures.

引用

页码：1608 / 1613

页数：6

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