Antagonistic involvement of FIS and H-NS proteins in the transcriptional control of hns expression

被引：81

作者：

Falconi, M ^{[1
]}

Brandi, A ^{[1
]}

LaTeana, A ^{[1
]}

Gualerzi, CO ^{[1
]}

Pon, CL ^{[1
]}

机构：

[1] UNIV CAMERINO, DEPT BIOL, GENET LAB, I-62032 CAMERINO, MC, ITALY

来源：

MOLECULAR MICROBIOLOGY | 1996年 / 19卷 / 05期

关键词：

D O I：

10.1046/j.1365-2958.1996.436961.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gel shift and DNase I footprinting experiments showed that Escherichia coli FIS (factor for inversion stimulation) protein binds to at least seven sites in the promoter region of hns. These sites extend from -282 to +25 with two sites, closely flanking the DNA bend located at -150 from the transcriptional startpoint, partly overlapping the H-NS binding sites involved in the transcriptional autorepression of hns. The interplay between FIS, H-NS and the hns promoter region were studied by examining the effects of FIS and H-NS on in vitro transcription of hns-cat fusions, as well as looking at the effect of FIS on preformed complexes containing H-NS and a DNA fragment derived from the hns promoter region. Taken together, our data suggest that in the cell, FIS and H-NS interact with the promoter region of hns and influence their respective interactions (possibly competing for the same binding site), eliciting antagonistic effects so that an interplay between these proteins might contribute to the transcriptional control of hns.

引用

页码：965 / 975

页数：11

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