Chemoenzymatic synthesis of glycosylphosphatidylinositol-anchored glycopeptides

被引：26

作者：

Wu, Zhimeng ^{[1
]}

Guo, Xueqing ^{[1
]}

Guo, Zhongwu ^{[1
]}

机构：

[1] Wayne State Univ, Dept Chem, Detroit, MI 48202 USA

来源：

CHEMICAL COMMUNICATIONS | 2010年 / 46卷 / 31期

关键词：

CHEMICAL-SYNTHESIS; SORTASE-A; PROTEIN; LIGATION; PEPTIDE; BIOSYNTHESIS;

D O I：

10.1039/c0cc00828a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

MUC1 glycopeptide was efficiently coupled to glycosylphosphatidylinositol (GPI) derivatives by sortase A (SrtA), verifying that SrtA can accept sterically hindered glycopeptide as substrate for ligation with GPIs. This work has established a practical method for the chemoenzymatic synthesis of GPI-linked glycopeptides.

引用

收藏

页码：5773 / 5774

页数：2

相关论文

共 20 条

[1] O-Glycosyl alpha-amino acids as building blocks for glycopeptide synthesis [J].

Arsequell, G ;

Valencia, G .

TETRAHEDRON-ASYMMETRY, 1997, 8 (17) :2839-2876

[2] Semisynthesis of a Glycosylphosphatidylinositol-Anchored Prion Protein [J].

Becker, Christian F. W. ;

Liu, Xinyu ;

Olschewski, Diana ;

Castelli, Riccardo ;

Seidel, Ralf ;

Seeberger, Peter H. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (43) :8215-8219

[3]

Cole R.N., 1997, N COMP BIOC B, P69

[4]

Eckert V., 1997, GLYCOSCIENCES, P223

[5]

FERGUSON MAJ, 1988, ANNU REV BIOCHEM, V57, P285

[6] Sortase-Catalyzed Peptide-Glycosylphosphatidylinositol Analogue Ligation [J].

Guo, Xueqing ;

Wang, Qianli ;

Swarts, Benjamin M. ;

Guo, Zhongwu .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (29) :9878-+

[7]

Hwa K, 2001, ADV EXP MED BIOL, V491, P207

[8] Sortase-mediated protein ligation: A new method for protein engineering [J].

Mao, HY ;

Hart, SA ;

Schink, A ;

Pollok, BA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (09) :2670-2671

[9] Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria [J].

Marraffini, LA ;

DeDent, AC ;

Schneewind, O .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2006, 70 (01) :192-+

[10] GPIomics: global analysis of glycosylphosphatidylinositol-anchored molecules of Trypanosoma cruzi [J].

Nakayasu, Ernesto S. ;

Yashunsky, Dmitry V. ;

Nohara, Lilian L. ;

Torrecilhas, Ana Claudia T. ;

Nikolaev, Andrei V. ;

Almeida, Igor C. .

MOLECULAR SYSTEMS BIOLOGY, 2009, 5