DNA-Molecular-Motor-Controlled Dendron Association

被引：30
|
作者：
Sun, Yawei [1 ]
Liu, Huajie [2 ]
Xu, Lijin [3 ]
Wang, Liying [1 ]
Fan, Qing-Hua [1 ]
Liu, Dongsheng [2 ]
机构：
[1] Chinese Acad Sci, Beijing Natl Lab Mol Sci, CAS Key Lab Mol Recognit & Funct, Inst Chem, Beijing 100190, Peoples R China
[2] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China
[3] Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China
来源：
LANGMUIR | 2010年 / 26卷 / 15期
基金：
中国国家自然科学基金;
关键词：
DENDRIMERS; NANOMACHINES;
D O I：
10.1021/la101802y
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
In this letter, we described a new strategy to study the macromolecule interactions rationally controlled by the movements of a DNA molecular motor. Two amphiphilic dendrons are covalently attached to the 3' and 5' ends of a pH-driven DNA motor, a 21-mer single-stranded DNA containing four stretches of cytosine-rich sequences. The resulting DNA-dendron conjugates were purified by polyacrylamide gel electrophoresis (PAGE), and their molecular weights were confirmed by MALDI-TOF. The reversible association-dissociation of the two DNA-attached dendrons controlled by the opening and closing of the DNA motor following pH changes was verified by circular dichroism spectroscopy and DNA stability studies in aqueous solutions. The results suggest that the DNA molecular motor may serve as a new platform for studying nonspecific and specific macromolecular interactions on the molecular level.
引用
收藏
页码：12496 / 12499
页数：4
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