Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection

被引：612

作者：

Stettler, Karin ^{[1
]}

Beltramello, Martina ^{[1
]}

Espinosa, Diego A. ^{[2
]}

Graham, Victoria ^{[3
]}

Cassotta, Antonino ^{[4
,5
]}

Bianchi, Siro ^{[1
]}

Vanzetta, Fabrizia ^{[1
]}

Minola, Andrea ^{[1
]}

Jaconi, Stefano ^{[1
]}

Mele, Federico

Foglierini, Mathilde ^{[4
]}

Pedotti, Mattia ^{[4
]}

Simonelli, Luca ^{[4
]}

Dowall, Stuart ^{[3
]}

Atkinson, Barry ^{[3
]}

Percivalle, Elena ^{[6
]}

Simmons, Cameron P. ^{[7
,8
,9
]}

Varani, Luca ^{[4
]}

Blum, Johannes ^{[10
,11
]}

Baldanti, Fausto ^{[6
]}

Cameroni, Elisabetta ^{[1
]}

Hewson, Roger

Harris, Eva ^{[2
]}

Lanzavecchia, Antonio ^{[4
,5
]}

Sallusto, Federica ^{[4
]}

Corti, Davide ^{[1
]}

机构：

[1] Humabs BioMed SA, Via Mirasole 1, CH-6500 Bellinzona, Switzerland

[2] Univ Calif Berkeley, Sch Publ Hlth, Div Infect Dis & Vaccinol, Berkeley, CA USA

[3] Publ Hlth England, Natl Infect Serv, Salisbury, Wilts, England

[4] Univ Svizzera Italiana, Inst Res Biomed, Via Vincenzo Vela 6, CH-6500 Bellinzona, Switzerland

[5] ETH, Inst Microbiol, Wolfgang Pauli Str 10, CH-8093 Zurich, Switzerland

[6] Fdn Ist Ricovero & Cura Carattere Sci IRCCS, Policlin San Matteo, Microbiol & Virol Dept, Mol Virol Unit, Pavia, Italy

[7] Univ Oxford, Nuffield Dept Med, Ctr Trop Med, Oxford OX1 2JD, England

[8] Univ Oxford, Clin Res Unit, Ctr Trop Med, Ho Chi Minh City, Vietnam

[9] Univ Melbourne, Peter Doherty Inst, Dept Microbiol & Immunol, 792 Elizabeth St, Melbourne, Vic 3000, Australia

[10] Swiss Trop & Publ Hlth Inst, Socinstr 57, CH-4002 Basel, Switzerland

[11] Univ Basel, Petersgraben 4, CH-4031 Basel, Switzerland

来源：

SCIENCE | 2016年 / 353卷 / 6301期

基金：

瑞士国家科学基金会; 美国国家卫生研究院; 欧洲研究理事会;

关键词：

DENGUE VIRUS; MONOCLONAL-ANTIBODIES; DETERMINANTS; ENHANCEMENT; EPITOPES; BINDING; FEVER; MODEL;

D O I：

10.1126/science.aaf8505

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Zika virus (ZIKV), a mosquito-borne flavivirus with homology to Dengue virus (DENV), has become a public health emergency. By characterizing memory lymphocytes from ZIKV-infected patients, we dissected ZIKV-specific and DENV-cross-reactive immune responses. Antibodies to nonstructural protein 1 (NS1) were largely ZIKV-specific and were used to develop a serological diagnostic tool. In contrast, antibodies against E protein domain I/II (EDI/II) were cross-reactive and, although poorly neutralizing, potently enhanced ZIKV and DENV infection in vitro and lethally enhanced DENV disease in mice. Memory T cells against NS1 or E proteins were poorly cross-reactive, even in donors preexposed to DENV. The most potent neutralizing antibodies were ZIKV-specific and targeted EDIII or quaternary epitopes on infectious virus. An EDIII-specific antibody protected mice from lethal ZIKV infection, illustrating the potential for antibody-based therapy.

引用

页码：823 / 826

页数：4

共 38 条

[1]

Aliota M. T., 2016, PLOS NEGLECT TROP D, V10

[2] The Human Immune Response to Dengue Virus Is Dominated by Highly Cross-Reactive Antibodies Endowed with Neutralizing and Enhancing Activity [J].

Beltramello, Martina ;

Williams, Katherine L. ;

Simmons, Cameron P. ;

Macagno, Annalisa ;

Simonelli, Luca ;

Quyen, Nguyen Than Ha ;

Sukupolvi-Petty, Soila ;

Navarro-Sanchez, Erika ;

Young, Paul R. ;

de Silva, Aravinda M. ;

Rey, Felix A. ;

Varani, Luca ;

Whitehead, Stephen S. ;

Diamond, Michael S. ;

Harris, Eva ;

Lanzavecchia, Antonio ;

Sallusto, Federica .

CELL HOST & MICROBE, 2010, 8 (03) :271-283

[3] Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study [J].

Calvet, Guilherme ;

Aguiar, Renato S. ;

Melo, Adriana S. O. ;

Sampaio, Simone A. ;

de Filippis, Ivano ;

Fabri, Allison ;

Araujo, Eliane S. M. ;

de Sequeira, Patricia C. ;

de Mendonca, Marcos C. L. ;

de Oliveira, Louisi ;

Tschoeke, Diogo A. ;

Schrago, Carlos G. ;

Thompson, Fabiano L. ;

Brasil, Patricia ;

dos Santos, Flavia B. ;

Nogueira, Rita M. R. ;

Tanurit, Amilcar ;

de Filippis, Ana M. B. .

LANCET INFECTIOUS DISEASES, 2016, 16 (06) :653-660

[4] Guillain-Barre Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study [J].

Cao-Lormeau, Van-Mai ;

Blake, Alexandre ;

Mons, Sandrine ;

Lastere, Stephane ;

Roche, Claudine ;

Vanhomwegen, Jessica ;

Dub, Timothee ;

Baudouin, Laure ;

Teissier, Anita ;

Larre, Philippe ;

Vial, Anne-Laure ;

Decam, Christophe ;

Choumet, Valerie ;

Halstead, Susan K. ;

Willison, Hugh J. ;

Musset, Lucile ;

Manuguerra, Jean-Claude ;

Despres, Philippe ;

Fournier, Emmanuel ;

Mallet, Henri-Pierre ;

Musso, Didier ;

Fontanet, Arnaud ;

Neil, Jean ;

Ghawche, Frederic .

LANCET, 2016, 387 (10027) :1531-1539

[5] Structures of the Zika Virus Envelope Protein and Its Complex with a Flavivirus Broadly Protective Antibody [J].

Dai, Lianpan ;

Song, Jian ;

Lu, Xishan ;

Deng, Yong-Qiang ;

Musyoki, Abednego Moki ;

Cheng, Huijun ;

Zhang, Yanfang ;

Yuan, Yuan ;

Song, Hao ;

Haywood, Joel ;

Xiao, Haixia ;

Yan, Jinghua ;

Shi, Yi ;

Qin, Cheng-Feng ;

Qi, Jianxun ;

Gao, George F. .

CELL HOST & MICROBE, 2016, 19 (05) :696-704

[6] A new class of highly potent, broadly neutralizing antibodies isolated from viremic patients infected with dengue virus [J].

Dejnirattisai, Wanwisa ;

Wongwiwat, Wiyada ;

Supasa, Sunpetchuda ;

Zhang, Xiaokang ;

Dai, Xinghong ;

Rouvinsky, Alexander ;

Jumnainsong, Amonrat ;

Edwards, Carolyn ;

Nguyen Than Ha Quyen ;

Duangchinda, Thaneeya ;

Grimes, Jonathan M. ;

Tsai, Wen-Yang ;

Lai, Chih-Yun ;

Wang, Wei-Kung ;

Malasit, Prida ;

Farrar, Jeremy ;

Simmons, Cameron P. ;

Zhou, Z. Hong ;

Rey, Felix A. ;

Mongkolsapaya, Juthathip ;

Screaton, Gavin R. .

NATURE IMMUNOLOGY, 2015, 16 (02) :170-+

[7]

Dowall S. D., 2016, PLOS NEGLECT TROP D, V10

[8] Zika Virus in the Americas - Yet Another Arbovirus Threat [J].

Fauci, Anthony S. ;

Morens, David M. .

NEW ENGLAND JOURNAL OF MEDICINE, 2016, 374 (07) :601-604

[9] Human naive and memory CD4+ T cell repertoires specific for naturally processed antigens analyzed using libraries of amplified T cells [J].

Geiger, Rebekka ;

Duhen, Thomas ;

Lanzavecchia, Antonio ;

Sallusto, Federica .

JOURNAL OF EXPERIMENTAL MEDICINE, 2009, 206 (07) :1525-1534

[10] Zika without symptoms in returning travellers: What are the implications? [J].

Ginier, Mylene ;

Neumayr, Andreas ;

Guenther, Stephan ;

Schmidt-Chanasit, Jonas ;

Blum, Johannes .

TRAVEL MEDICINE AND INFECTIOUS DISEASE, 2016, 14 (01) :16-20

← 1 2 3 4 →