Derivatization of peptides as quaternary ammonium salts for sensitive detection by ESI-MS

被引：37

作者：

Cydzik, Marzena ^{[1
]}

Rudowska, Magdalena ^{[1
]}

Stefanowicz, Piotr ^{[1
]}

Szewczuk, Zbigniew ^{[1
]}

机构：

[1] Univ Wroclaw, Fac Chem, PL-50383 Wroclaw, Poland

来源：

JOURNAL OF PEPTIDE SCIENCE | 2011年 / 17卷 / 06期

关键词：

quaternary ammonium salts; derivatization of peptides; peptide fragmentation; sequencing; TANDEM MASS-SPECTROMETRY; COLLISION-INDUCED DISSOCIATION; TERMINAL DERIVATIZATION; CHARGE DERIVATIZATION; PROTONATED PEPTIDES; AMADORI PRODUCTS; FRAGMENTATION; DERIVATIVES; IONS;

D O I：

10.1002/psc.1342

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of model peptides in the form of quaternary ammonium salts at the N-terminus was efficiently prepared by the solid-phase synthesis. Tandem mass spectrometric analysis of the peptide quaternary ammonium derivatives was shown to provide sequence confirmation and enhanced detection. We designed the 2-(1,4-diazabicyclo[2.2.2] octylammonium) acetyl quaternary ammonium group which does not suffer from neutral losses during MS/MS experiments. The presented quaternization of 1,4-diazabicyclo[2.2.2] octane (DABCO) by iodoacetylated peptides is relatively easy and compatible with standard solid-phase peptide synthesis. This methodology offers a novel sensitive approach to analyze peptides and other compounds. Copyright (C) 2011 European Peptide Society and John Wiley & Sons, Ltd.

引用

页码：445 / 453

页数：9

共 37 条

[1] CHARGE-REMOTE FRAGMENTATIONS - ANALYTICAL APPLICATIONS AND FUNDAMENTAL-STUDIES [J].