Large-scale clinical validation of a lateral flow immunoassay for detection of cryptococcal antigen in serum and cerebrospinal fluid specimens

被引：11

作者：

Suwantarat, Nuntra ^{[1
]}

Dalton, Justin B. ^{[1
]}

Lee, Richard ^{[2
]}

Green, Rachel ^{[2
]}

Memon, Warda ^{[2
]}

Carroll, Karen C. ^{[1
,2
]}

Riedel, Stefan ^{[1
,3
]}

Zhang, Sean X. ^{[1
,2
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Pathol, Div Med Microbiol, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ Hosp, Microbiol Lab, Baltimore, MD 21287 USA

[3] Johns Hopkins Bayview Med Ctr, Baltimore, MD USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2015年 / 82卷 / 01期

关键词：

Cryptococcus; Antigen; Lateral flow immunoassay; Cerebrospinal fluid; Serum; GATTII SEROTYPE-C; AIDS PATIENTS; MENINGITIS; NEOFORMANS; PREVALENCE; AFRICA;

D O I：

10.1016/j.diagmicrobio.2015.01.012

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We compared a lateral flow immunoassay (LFA) to a currently used enzyme immunoassay for detection of cryptococcal antigen in 396 sera and 651 cerebrospinal fluid specimens. We found 97% concordance between the 2 assays. The LFA detected an additional 22 positives. Overall, the LFA had sensitivity of 100% and specificity of 99.6% for the diagnosis of cryptococcosis. The LFA is rapid, accurate, and easy to perform, and it is suitable for routine patient care testing. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：54 / 56

页数：3

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