A comparison of four commercially available RNA extraction kits for wastewater surveillance of SARS-CoV-2 in a college population

被引:13
作者
O'Brien, Megan [1 ]
Rundell, Zachary C. [2 ]
Nemec, Michelle D. [1 ]
Langan, Laura M. [3 ]
Back, Jeffrey A. [2 ]
Lugo, Joaquin N. [4 ]
机构
[1] Baylor Univ, Mol Biosci Ctr, Waco, TX 76798 USA
[2] Baylor Univ, Ctr Reservoir & Aquat Syst, Waco, TX 76798 USA
[3] Baylor Univ, Dept Environm Sci, Waco, TX 76798 USA
[4] Baylor Univ, Dept Psychiat & Neurosci, 1 Bear Pl 97334, Waco, TX 76798 USA
关键词
Wastewater-based epidemiology; WBE; sewage; COVID-19;
D O I
10.1016/j.scitotenv.2021.149595
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
processing times between approximately 9 and 9.5 h. Based on the current research, the most effective kits for smaller population densities are pellet based and include a homogenization, inhibitor removal, and RNA preser-vation step. (c) 2021 Elsevier B.V. All rights reserved.
引用
收藏
页数:6
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