A comparison of four commercially available RNA extraction kits for wastewater surveillance of SARS-CoV-2 in a college population

被引：13
作者：
O'Brien, Megan [1 ]
Rundell, Zachary C. [2 ]
Nemec, Michelle D. [1 ]
Langan, Laura M. [3 ]
Back, Jeffrey A. [2 ]
Lugo, Joaquin N. [4 ]
机构：
[1] Baylor Univ, Mol Biosci Ctr, Waco, TX 76798 USA
[2] Baylor Univ, Ctr Reservoir & Aquat Syst, Waco, TX 76798 USA
[3] Baylor Univ, Dept Environm Sci, Waco, TX 76798 USA
[4] Baylor Univ, Dept Psychiat & Neurosci, 1 Bear Pl 97334, Waco, TX 76798 USA
来源：
SCIENCE OF THE TOTAL ENVIRONMENT | 2021年 / 801卷
关键词：
Wastewater-based epidemiology; WBE; sewage; COVID-19;
D O I：
10.1016/j.scitotenv.2021.149595
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
processing times between approximately 9 and 9.5 h. Based on the current research, the most effective kits for smaller population densities are pellet based and include a homogenization, inhibitor removal, and RNA preser-vation step. (c) 2021 Elsevier B.V. All rights reserved.
引用
收藏
页数：6
相关论文
共 50 条
  • [1] Wastewater Surveillance for SARS-CoV-2 RNA in Canada
    Hrudey, Steve E.
    Bischel, Heather N.
    Charrois, Jeff
    Chik, Alex H. S.
    Conant, Bernadette
    Delatolla, Rob
    Dorner, Sarah
    Graber, Tyson E.
    Hubert, Casey
    Isaac-Renton, Judy
    Pons, Wendy
    Safford, Hannah
    Servos, Mark
    Sikora, Christopher
    FACETS, 2022, 7 : 1493 - 1597
  • [2] Wastewater Surveillance of SARS-CoV-2 RNA in a Prison Facility
    Thakali, Ocean
    Shahin, Shalina
    Sherchan, Samendra P.
    WATER, 2024, 16 (04)
  • [3] Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA
    Ute Eberle
    Clara Wimmer
    Ingrid Huber
    Antonie Neubauer-Juric
    Giuseppe Valenza
    Nikolaus Ackermann
    Andreas Sing
    European Journal of Clinical Microbiology & Infectious Diseases, 2021, 40 : 1303 - 1308
  • [4] Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA
    Eberle, Ute
    Wimmer, Clara
    Huber, Ingrid
    Neubauer-Juric, Antonie
    Valenza, Giuseppe
    Ackermann, Nikolaus
    Sing, Andreas
    EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2021, 40 (06) : 1303 - 1308
  • [5] Coprostanol as a Population Biomarker for SARS-CoV-2 Wastewater Surveillance Studies
    Reynolds, Liam J.
    Sala-Comorera, Laura
    Khan, Mohd Faheem
    Martin, Niamh A.
    Whitty, Megan
    Stephens, Jayne H.
    Nolan, Tristan M.
    Joyce, Eadaoin
    Fletcher, Nicola F.
    Murphy, Cormac D.
    Meijer, Wim G.
    WATER, 2022, 14 (02)
  • [6] Surveillance of SARS-CoV-2 RNA in wastewater matrix: a review
    Nitish Venkateswarlu Mogili
    Maheswara Reddy Mallu
    Jagadeeshwar Kodavaty
    Rajeswara Reddy Erva
    Environmental Monitoring and Assessment, 2024, 196
  • [7] Surveillance of SARS-CoV-2 RNA in wastewater matrix: a review
    Mogili, Nitish Venkateswarlu
    Mallu, Maheswara Reddy
    Kodavaty, Jagadeeshwar
    Erva, Rajeswara Reddy
    ENVIRONMENTAL MONITORING AND ASSESSMENT, 2024, 196 (01)
  • [8] Wastewater-based SARS-CoV-2 surveillance and sequencing
    Michie, Alice
    MICROBIOLOGY AUSTRALIA, 2024, 45 (01) : 8 - 12
  • [9] Comparison of kits for SARS-CoV-2 extraction in liquid and passive samples
    Lau, Melody
    King, Brendon J.
    Keegan, Alexandra
    Drigo, Barbara
    Donner, Erica
    Monis, Paul
    LETTERS IN APPLIED MICROBIOLOGY, 2023, 76 (12)
  • [10] SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters
    Sovova, Katerina
    Vasickova, Petra
    Valasek, Vojtech
    Vyravsky, David
    Ocenaskov, Vera
    Juranov, Eva
    Busova, Milena
    Tucek, Milan
    Bencko, Vladimir
    Mlejnkova, Hana Zverinova
    WATER RESEARCH X, 2024, 23
12345