Tanshinone II-A induced apoptosis of HepG2: involvement of p53, Bcl-2 and Bax

被引：0

作者：

Wang, Pingqing ^{[1
]}

Li, Zhizhong ^{[1
]}

Ouyang, Keqing ^{[1
]}

Qin, Zhihui ^{[1
]}

He, Xi ^{[1
]}

Long, Ruicai ^{[1
]}

Yan, Yan ^{[1
]}

机构：

[1] Chongqing Univ, Coll Bioengn, Chongqing 400044, Peoples R China

来源：

2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11 | 2009年

关键词：

Tanshinone II-A; Cell proliferation; Apoptosis; Cell cycle; CANCER-CELLS; MEDICINE;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Background: Tanshinone was thought to be a potential antitumor drug. In this study, we investigated the apoptosis of hepatoma HepG2 induced by Tanshinone II-A as well as the involved molecules. Methods: The inhibitory effects on cell proliferation of Tanshinone II-A were assessed by MTT method. DNA fragmentation was evaluated by agarose gel electrophoresis. Cell cycle and apoptotic rates were quantified by flow cytometry (FCM). The expressions of p53, Bcl-2, Bax genes were determined by semi-quantitative RT-PCR. Results: Tanshinone II-A significantly inhibited the proliferation rate of human hepatoma HepG2 cells in dose- and time-dependent manner. The semi-inhibitory concentration (IC(50)) was 7.4 mu g/ml, 1.9 mu g/ml, 0.6 mu g/ml for 24, 48, and 72 h respectively. DNA fragmentation in agarose gel revealed series of DNA ladder, which indicated the degradation of genomic DNA induced by apoptosis. Flow cytometry showed that hepatoma HepG2 cells were block in G0/G1 phase and the apoptotic rate elevated significantly after treatment with Tanshinone II-A. In addition, semi-quantitative RT-PCR showed that p53 and Bax increased significantly while Bcl-2 decreased significantly in HepG2 treated with Tanshinone II-A. Conclusion: Tanshinone II-A could inhibit proliferation and induce apoptosis of the human hepatoma HepG2 cells. p53, Bcl-2 and Bax might be involved in this process.

引用

页码：2968 / 2971

页数：4

共 50 条

[21] bcl-2 expression, p53 accumulation, and apoptosis in ovarian carcinomas [J].

Diebold, J ;

Baretton, G ;

Felchner, M ;

Meier, W ;

Dopfer, K ;

Schmidt, M ;

Lohrs, U .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1996, 105 (03) :341-349

[22] The prognostic value of spontaneous apoptosis, Bax, Bcl-2, and p53 in oral squamous cell carcinoma of the tongue [J].

Xie, X ;

Clausen, OPF ;

De Angelis, P ;

Boysen, M .

CANCER, 1999, 86 (06) :913-920

[23] Apoptosis in thyroid neoplasms: relationship with p53 and bcl-2 expression [J].

Moore, D ;

Ohene-Fianko, D ;

Garcia, B ;

Chakrabarti, S .

HISTOPATHOLOGY, 1998, 32 (01) :35-42

[24] Apoptosis and cell cycle aberrations in epithelial odontogenic lesions: An evidence by the expression of p53, Bcl-2 and Bax [J].

Tenorio, Jefferson-da Rocha ;

Santana, Thalita ;

Queiroz, Salomao-Israel-Monteiro-Lourenco ;

de Oliveira, Denise-Helen-Imaculada-Pereira ;

Queiroz, Lelia-Maria-Guedes .

MEDICINA ORAL PATOLOGIA ORAL Y CIRUGIA BUCAL, 2018, 23 (02) :E120-E125

[25] Decursin induces apoptosis by regulating AMP-activated protein kinase and Bax/Bcl-2 pathway in HepG2 cell line [J].

Ravinayagam, Vijaya ;

Shehzad, Adeeb ;

Almohazey, Dana ;

Almofty, Sarah ;

Aljafary, Meneerah A. ;

Alhamed, Nada A. ;

Alhamed, Najla ;

Al-Rashid, Noor A. ;

Al-Suhaimi, Ebtesam A. .

EUROPEAN JOURNAL OF INTEGRATIVE MEDICINE, 2018, 24 :17-22

[26] Expression of p53, bcl-2, bax, bcl-x2 and c-myc in radiation-induced apoptosis in Burkitt's lymphoma cells [J].

Khanna, KK ;

Wie, T ;

Song, QZ ;

Burrows, SR ;

Moss, DJ ;

Krajewski, S ;

Reed, JC ;

Lavin, MF .

CELL DEATH AND DIFFERENTIATION, 1996, 3 (03) :315-322

[27] Phenylbutyrate-induced apoptosis and differential expression of Bcl-2, Bax, p53 and Fas in human prostate cancer cell lines [J].

Ng, AY ;

Bales, W ;

Veltri, RW .

ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY, 2000, 22 (01) :45-54

[28] Drug-induced apoptosis and p53, BCL-2 and BAX expression in breast cancer tissues in vivo and in fibroblast cells in vitro [J].

Suzuki, K ;

Kazui, T ;

Yoshida, M ;

Uno, T ;

Kobayashi, T ;

Kimura, T ;

Yoshida, T ;

Sugimura, H .

JAPANESE JOURNAL OF CLINICAL ONCOLOGY, 1999, 29 (07) :323-331

[29] Bromocriptine induced apoptosis in pituitary adenoma cells: relationship to p53 and bcl-2 expression [J].

Yin, DL ;

Tamaki, N ;

Kokunai, T ;

Yasuo, K ;

Yonezawa, K .

JOURNAL OF CLINICAL NEUROSCIENCE, 1999, 6 (04) :326-331

[30] Deltamethrin induces altered expression of P53, Bax and Bcl-2 in rat brain [J].

Wu, AG ;

Ren, TL ;

Hu, QS ;

Liu, YG .

NEUROSCIENCE LETTERS, 2000, 284 (1-2) :29-32

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