Bioelectrochemical hydrogen production with hydrogenophilic dechlorinating bacteria as electrocatalytic agents

被引:65
作者
Villano, Marianna [1 ]
De Bonis, Luca [1 ]
Rossetti, Simona [2 ]
Aulenta, Federico [1 ]
Majone, Mauro [1 ]
机构
[1] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
[2] CNR, Water Res Inst IRSA CNR, Area Ric Roma Montelibretti 1, I-00015 Monterotondo, RM, Italy
关键词
Bioelectrochemical systems; Biocathode; Dechlorinating bacteria; Hydrogen production; Redox mediator; MICROBIAL ELECTROLYSIS CELLS; FUEL-CELLS; STAINLESS-STEEL; GENOME SEQUENCE; CATHODES; TETRACHLOROETHENE; EVOLUTION; ETHENE; CULTURE; ENZYMES;
D O I
10.1016/j.biortech.2010.10.146
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Hydrogenophilic dechlorinating bacteria were shown to catalyze H-2 production by proton reduction, with electrodes serving as electron donors, either in the presence or in the absence of a redox mediator. In the presence of methyl viologen, Desulfitobacterium- and Dehalococcoides-enriched cultures produced H-2 at rates as high as 12.4 mu eq/mgVSS (volatile suspended solids)/d, with the cathode set at -450 mV vs. the standard hydrogen electrode (SHE), hence very close to the reversible H+/H-2 potential value of -414 mV at pH 7. Notably, the Desulfitobacterium-enriched culture was capable of catalyzing H-2 production without mediators at cathode potentials lower than -700 mV. At -750 mV, the H-2 production rate with Desulfitobacterium spp. was 13.5 mu eq/mgVSS/d (or 16 mu eq/cm(2)/d), nearly four times higher than that of the abiotic controls. Overall, this study suggests the possibility of employing dechlorinating bacteria as hydrogen catalysts in new energy technologies such as microbial electrolysis cells. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3193 / 3199
页数:7
相关论文
共 39 条
[1]   The anode potential regulates bacterial activity in microbial fuel cells [J].
Aelterman, Peter ;
Freguia, Stefano ;
Keller, Jurg ;
Verstraete, Willy ;
Rabaey, Korneel .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 78 (03) :409-418
[2]  
[Anonymous], 1995, Standard methods for examination of water and waste water, V19th
[3]   Dynamic electrochemical investigations of hydrogen oxidation and production by enzymes and implications for future technology [J].
Armstrong, Fraser A. ;
Belsey, Natalie A. ;
Cracknell, James A. ;
Goldet, Gabrielle ;
Parkin, Alison ;
Reisner, Erwin ;
Vincent, Kylie A. ;
Wait, Annemarie F. .
CHEMICAL SOCIETY REVIEWS, 2009, 38 (01) :36-51
[4]   Comparative study of methanol, butyrate, and hydrogen as electron donors for long-term dechlorination of tetrachloroethene in mixed anerobic cultures [J].
Aulenta, F ;
Gossett, JM ;
Papini, MP ;
Rossetti, S ;
Majone, M .
BIOTECHNOLOGY AND BIOENGINEERING, 2005, 91 (06) :743-753
[5]   Complete dechlorination of tetrachloroethene to ethene in presence of methanogenesis and acetogenesis by an anaerobic sediment microcosm [J].
Aulenta, F ;
Majone, M ;
Verbo, P ;
Tandoi, V .
BIODEGRADATION, 2002, 13 (06) :411-424
[6]   Trichloroethene dechlorination and H2 evolution are alternative biological pathways of electric charge utilization by a dechlorinating culture in a bioelectrochemical system [J].
Aulenta, Federico ;
Canosa, Andrea ;
Majone, Mauro ;
Panero, Stefania ;
Reale, Priscilla ;
Rossetti, Simona .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2008, 42 (16) :6185-6190
[7]   Hydrogen Production by a Hyperthermophilic Membrane-Bound Hydrogenase in Water-Soluble Nanolipoprotein Particles [J].
Baker, Sarah E. ;
Hopkins, Robert C. ;
Blanchette, Craig D. ;
Walsworth, Vicki L. ;
Sumbad, Rhoda ;
Fischer, Nicholas O. ;
Kuhn, Edward A. ;
Coleman, Matt ;
Chromy, Brett A. ;
Letant, Sonia E. ;
Hoeprich, Paul D. ;
Adams, Michael W. W. ;
Henderson, Paul T. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (22) :7508-+
[8]   METHANOGENS - RE-EVALUATION OF A UNIQUE BIOLOGICAL GROUP [J].
BALCH, WE ;
FOX, GE ;
MAGRUM, LJ ;
WOESE, CR ;
WOLFE, RS .
MICROBIOLOGICAL REVIEWS, 1979, 43 (02) :260-296
[9]   High Surface Area Stainless Steel Brushes as Cathodes in Microbial Electrolysis Cells [J].
Call, Douglas F. ;
Merrill, Matthew D. ;
Logan, Bruce E. .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2009, 43 (06) :2179-2183
[10]   Enzymes as working or inspirational electrocatalysts for fuel cells and electrolysis [J].
Cracknell, James A. ;
Vincent, Kylie A. ;
Armstrong, Fraser A. .
CHEMICAL REVIEWS, 2008, 108 (07) :2439-2461