MiR-193b regulates early chondrogenesis by inhibiting the TGF-beta2 signaling pathway

被引：61

作者：

Hou, Changhe ^{[1
]}

Yang, Zibo ^{[1
]}

Kang, Yan ^{[1
]}

Zhang, Ziji ^{[1
]}

Fu, Ming ^{[1
]}

He, Aishan ^{[1
]}

Zhang, Zhiqi ^{[1
]}

Liao, Weiming ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Joint Dept, Affiliated Hosp 1, Guangzhou 510080, Guangdong, Peoples R China

来源：

FEBS LETTERS | 2015年 / 589卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Chondrogenesis; Osteoarthritis; MiR-193b; TGF-beta; TNF-alpha; REPRESSES CELL-PROLIFERATION; GROWTH-FACTOR-BETA; CHONDROCYTE PROLIFERATION; STEM-CELLS; OSTEOGENIC DIFFERENTIATION; ARTICULAR-CARTILAGE; EXPRESSION; MICRORNAS; RUNX2; SOX9;

D O I：

10.1016/j.febslet.2015.02.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cartilage generation and degradation are regulated by miRNAs. Our previous study has shown altered expression of miR-193b in chondrogenic human adipose-derived mesenchymal stem cells (hADSCs). In the current study, we investigated the role of miR-193b in chondrogenesis and cartilage degradation. Luciferase reporter assays showed that miR-193b targeted seed sequences of the TGFB2 and TGFBR3 3'-UTRs. MiR-193b suppressed the expression of early chondrogenic markers in chondrogenic ATDC5 cells, and TNF-alpha expression in IL-1b-induced PMCs. In conclusion, MiR-193b may inhibit early chondrogenesis by targeting TGFB2 and TGFBR3, and may regulate inflammation by repressing TNF-alpha expression in inflamed chondrocytes. (C) 2015 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：1040 / 1047

页数：8

共 59 条

[1] Inflammation in osteoarthritis [J].

Goldring, Mary B. ;

Otero, Miguel .

CURRENT OPINION IN RHEUMATOLOGY, 2011, 23 (05) :471-478

[2] Control of chondrogenesis by the transcription factor Sox9 [J].

Akiyama, Haruhiko .

MODERN RHEUMATOLOGY, 2008, 18 (03) :213-219

[3] ECM stiffness primes the TGFβ pathway to promote chondrocyte differentiation [J].

Allen, Jessica L. ;

Cooke, Margaret E. ;

Alliston, Tamara .

MOLECULAR BIOLOGY OF THE CELL, 2012, 23 (18) :3731-3742

[4] Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [J].

Arner, Erik ;

Mejhert, Niklas ;

Kulyte, Agne ;

Balwierz, Piotr J. ;

Pachkov, Mikhail ;

Cormont, Mireille ;

Lorente-Cebrian, Silvia ;

Ehrlund, Anna ;

Laurencikiene, Jurga ;

Heden, Per ;

Dahlman-Wright, Karin ;

Tanti, Jean-Francois ;

Hayashizaki, Yoshihide ;

Ryden, Mikael ;

Dahlman, Ingrid ;

van Nimwegen, Erik ;

Daub, Carsten O. ;

Arner, Peter .

DIABETES, 2012, 61 (08) :1986-1993

[5] The Regulation of Differentiation in Mesenchymal Stem Cells [J].

Augello, Andrea ;

De Bari, Cosimo .

HUMAN GENE THERAPY, 2010, 21 (10) :1226-1238

[6]

Bian Qin, 2012, Front Biosci (Elite Ed), V4, P74

[7] Osteoarthritis: can anti-cytokine therapy play a role in treatment? [J].

Calich, Ana Luisa G. ;

Domiciano, Diogo S. ;

Fuller, Ricardo .

CLINICAL RHEUMATOLOGY, 2010, 29 (05) :451-455

[8] Chondrocyte-intrinsic Smad3 represses runx2-inducible matrix metalloproteinase 13 expression to maintain articular cartilage and prevent osteoarthritis [J].

Chen, Carol G. ;

Thuillier, Daniel ;

Chin, Emily N. ;

Alliston, Tamara .

ARTHRITIS AND RHEUMATISM, 2012, 64 (10) :3278-3289

[9] Runx2 Regulates Endochondral Ossification Through Control of Chondrocyte Proliferation and Differentiation [J].

Chen, Haiyan ;

Ghori-Javed, Farah Y. ;

Rashid, Harunur ;

Adhami, Mitra D. ;

Serra, Rosa ;

Gutierrez, Soraya E. ;

Javed, Amjad .

JOURNAL OF BONE AND MINERAL RESEARCH, 2014, 29 (12) :2653-2665

[10] MicroRNA-193b Represses Cell Proliferation and Regulates Cyclin D1 in Melanoma [J].

Chen, Jiamin ;

Feilotter, Harriet E. ;

Pare, Genevieve C. ;

Zhang, Xiao ;

Pemberton, Joshua G. W. ;

Garady, Cherif ;

Lai, Dulcie ;

Yang, Xiaolong ;

Tron, Victor A. .

AMERICAN JOURNAL OF PATHOLOGY, 2010, 176 (05) :2520-2529

← 1 2 3 4 5 6 →