Systemic inactivation and phenotypic characterization of two-component systems in expression of Streptococcus mutans virulence properties

被引：61
作者：
Levesque, C. M. [1 ]
Mair, R. W. [1 ]
Perry, J. A. [1 ]
Lau, P. C. Y. [1 ]
Li, Y.-H. [1 ]
Cvitkovitch, D. G. [1 ]
机构：
[1] Univ Toronto, Fac Dent, Inst Dent Res, Toronto, ON M5G 1G6, Canada
来源：
LETTERS IN APPLIED MICROBIOLOGY | 2007年 / 45卷 / 04期
关键词：
acid resistance; biofilm formation; gene inactivation; oral streptococci; stress tolerance; two component system;
D O I：
10.1111/j.1472-765X.2007.02203.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aims: To assess potential function of each two-component signal transduction system in the expression of Streptococcus mutans virulence properties. Methods and Results: For each two-component system (TCS), the histidine kinase-encoding gene was inactivated by a polymerase chain reaction (PCR)-based deletion strategy and the effects of gene disruption on the cell's ability to form biofilms, become competent, and tolerate acid, osmotic, and oxidative stress conditions were tested. Our results demonstrated that none of the mutations were lethal for S. mutans. The TCS-2 (CiaRH) is involved in biofilm formation and tolerance to environmental stresses, the TCS-3 (ScnRK-like) participates in the survival of cells at acidic pH, and the TCS-9 affects the acid tolerance response and the process of streptococcal competence development. Conclusion: Our results confirmed the physiological role of the TCS in S. mutans cellular function, in particular the SncRK-like TCS and TCS-9 as they may represent new regulatory systems than can be involved in S. mutans pathogenesis. Significance and Impact of the study: Multiple TCS govern important biological parameters of S. mutans enabling its survival and persistence in the biofilm community.
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页码：398 / 404
页数：7
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