Improvement of catalytic activity of lipase in the presence of calix[4]arene valeric acid or hydrazine derivative

被引:7
作者
Akoz, Enise [1 ]
Sayin, Serkan [1 ]
Kaplan, Selcuk [1 ]
Yilmaz, Mustafa [1 ]
机构
[1] Selcuk Univ, Dept Chem, TR-42031 Konya, Turkey
关键词
Nanoparticles; Fe3O4; Lipase; Calixarene; Enantioselectivity; CANDIDA-RUGOSA LIPASE; RACEMIC NAPROXEN; CROWN-ETHERS; ENANTIOSELECTIVITY; IMMOBILIZATION; HYDROLYSIS; ENHANCEMENT; SELECTIVITY; REGULATORS;
D O I
10.1007/s00449-014-1299-x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Sol-gel encapsulation is a simple but powerful method to enhance the enantioselectivity of lipase-catalyzed transformations in an isooctane/aqueous buffer solution. Candida rugosa lipase was encapsulated according to a sol-gel procedure in the presence and absence of calix[4]arene hydrazine or carboxylic acid derivatives with Fe3O4 magnetic nanoparticles as an additive. The activity of the encapsulated lipases was evaluated for the enantioselective hydrolysis of racemic Naproxen methyl ester and the hydrolysis of p-Nitrophenylpalmitate. The results indicate that the encapsulated lipase without calix[4]arene derivative has lower conversion and enantioselectivity compared to the encapsulated lipase with calix[4]arene derivative. It was found that the calix[4]arene hydrazine and carboxylic acid-based encapsulated lipases have excellent activity and enantioselectivity (E > 300) compared to encapsulated lipase without the calix[4]arene derivatives.
引用
收藏
页码:595 / 604
页数:10
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