Chicken-derived RSPO1 and WNT3 contribute to maintaining longevity of chicken intestinal organoid cultures

被引:13
作者
Oost, Miriam J. [1 ]
Ijaz, Adil [2 ]
van Haarlem, Daphne A. [2 ]
van Summeren, Kitty [3 ]
Velkers, Francisca C. [4 ]
Kraneveld, Aletta D. [5 ]
Venema, Koen [1 ]
Jansen, Christine A. [2 ,6 ]
Pieters, Raymond H. H. [3 ,7 ]
ten Klooster, Jean Paul [3 ]
机构
[1] Maastricht Univ, Fac Sci & Engn, Ctr Hlth Eating & Food Innovat, Campus Venlo, Venlo, Netherlands
[2] Univ Utrecht, Fac Vet Med, Dept Biomol Hlth Sci, Div Infect Dis & Immunol, Utrecht, Netherlands
[3] Univ Appl Sci Utrecht, Res Ctr Hlth & Sustainable Living, Innovat Testing Life Sci & Chem, Utrecht, Netherlands
[4] Univ Utrecht, Fac Vet Med, Dept Populat Hlth Sci, Div Farm Anim Hlth, Utrecht, Netherlands
[5] Univ Utrecht, Fac Sci, Utrecht Inst Pharmaceut Sci, Div Pharmacol, Utrecht, Netherlands
[6] Wageningen Univ & Res, Dept Anim Sci, Cell Biol & Immunol Grp, Wageningen, Netherlands
[7] Univ Utrecht, Inst Risk Assessment Sci, Dept Populat Hlth Sci, Utrecht, Netherlands
基金
荷兰研究理事会;
关键词
IN-VITRO; STEM-CELLS; EXPANSION; FOXO1;
D O I
10.1038/s41598-022-14875-7
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Intestinal organoids are advanced cellular models, which are widely used in mammalian studies to mimic and study in vivo intestinal function and host-pathogen interactions. Growth factors WNT3 and RSPO1 are crucial for the growth of intestinal organoids. Chicken intestinal organoids are currently cultured with mammalian Wnt3a and Rspo1, however, maintaining their longevity has shown to be challenging. Based on the limited homology between mammalian and avian RSPO1, we expect that chicken-derived factors are required for the organoid cultures. Isolated crypts from embryonic tissue of laying hens were growing in the presence of chicken WNT3 and RSPO1, whereas growth in the presence of mammalian Wnt3a and Rspo1 was limited. Moreover, the growth was increased by using Prostaglandin E2 (PGE(2)) and a Forkhead box O1-inhibitor (FOXO1-inhibitor), allowing to culture these organoids for 15 passages. Furthermore, stem cells maintained their ability to differentiate into goblets, enterocytes and enteroendocrine cells in 2D structures. Overall, we show that chicken intestinal organoids can be cultured for multiple passages using chicken-derived WNT3 and RSPO1, PGE(2), and FOXO1-inhibitor.
引用
收藏
页数:12
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