Non-invasive Transdermal Delivery of Human Insulin Using Ionic Liquids: In vitro Studies

被引：57

作者：

Jorge, Ludmilla R. ^{[1
]}

Harada, Liliam K. ^{[1
]}

Silva, Erica C. ^{[1
]}

Campos, Welida F. ^{[1
]}

Morelli, Fernanda C. ^{[1
]}

Shimamoto, Gustavo ^{[2
]}

Pereira, Jorge F. B. ^{[3
]}

Oliveira Jr, Jose M. ^{[1
]}

Tubino, Matthieu ^{[2
]}

Vila, Marta M. D. C. ^{[1
]}

Balcao, Victor M. ^{[1
,4
,5
]}

机构：

[1] Univ Sorocaba, Lab Biofilms & Bacteriophages, PhageLab, Sorocaba, Brazil

[2] Univ Estadual Campinas, Inst Chem, Campinas, Brazil

[3] Univ Coimbra, CIEPOPF Dept Chem Engn, Coimbra, Portugal

[4] Univ Aveiro, Dept Biol, Aveiro, Portugal

[5] Univ Aveiro, CESAM, Aveiro, Portugal

来源：

FRONTIERS IN PHARMACOLOGY | 2020年 / 11卷

基金：

巴西圣保罗研究基金会;

关键词：

human insulin; ionic liquids; choline geranate; choline oleate; transdermal permeation; chemical and biological characterization; EXTRACTION; STABILITY; SYSTEMS;

D O I：

10.3389/fphar.2020.00243

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In this research project, synthesis and characterization of ionic liquids and their subsequent utilization as facilitators of transdermal delivery of human insulin was pursued. Choline geranate and choline oleate ionic liquids (and their deep eutectic solvents) were produced and characterized by nuclear magnetic resonance (H-1 NMR), water content, oxidative stability, cytotoxicity and genotoxicity assays, and ability to promote transdermal protein permeation. The results gathered clearly suggest that all ionic liquids were able to promote/facilitate transdermal permeation of insulin, although to various extents. In particular, choline geranate 1:2 combined with its virtually nil cyto- and geno-toxicity was chosen to be incorporated in a biopolymeric formulation making it a suitable facilitator aiming at transdermal delivery of insulin.

引用

页数：17

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