Curcumin attenuates osteogenic differentiation and calcification of rat vascular smooth muscle cells

被引:33
|
作者
Hou, Menglin [1 ]
Song, Yan [1 ]
Li, Zhenlin [2 ]
Luo, Chufan [3 ]
Ou, Jing-Song [4 ,5 ]
Yu, Huimin [6 ,7 ]
Yan, Jianyun [2 ]
Lu, Lihe [1 ]
机构
[1] Sun Yat Sen Univ, Zhongshan Med Sch, Dept Pathophysiolgy, 74 Zhongshan Er Rd, Guangzhou 510080, Guangdong, Peoples R China
[2] Southern Med Univ, Dept Histol & Embryol, Key Lab Tissue Construct & Detect Guangdong Prov, Guangzhou, Guangdong, Peoples R China
[3] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Cardiol, Guangzhou, Guangdong, Peoples R China
[4] Sun Yat Sen Univ, Affiliated Hosp 1, Div Cardiac Surg, Guangzhou, Guangdong, Peoples R China
[5] Sun Yat Sen Univ, Key Lab Assisted Circulat, Minist Hlth, Guangzhou, Guangdong, Peoples R China
[6] Guangdong Acad Med Sci, Guangdong Gen Hosp, Dept Cardiol, Guangzhou, Guangdong, Peoples R China
[7] Guangdong Cardiovasc Inst, Guangzhou, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
Curcumin; Vascular smooth muscle cells; Calcification; Apoptosis; IN-VITRO; MATRIX MINERALIZATION; EXTRACELLULAR CALCIUM; INDUCED INFLAMMATION; KINASE JNK; APOPTOSIS; PHOSPHATE; INHIBITION; PATHWAY; VIVO;
D O I
10.1007/s11010-016-2778-y
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Vascular calcification has been considered as a biological process resembling bone formation involving osteogenic differentiation. It is a major risk factor for cardiovascular morbidity and mortality. Previous studies have shown the protective effects of curcumin on cardiovascular diseases. However, whether curcumin has effects on osteogenic differentiation and calcification of vascular smooth muscle cells (VSMCs) has not been reported. In the present study, we used an in vitro model of VSMC calcification to investigate the role of curcumin in the progression of rat VSMC calcification. Curcumin treatment significantly reduced calcification of VSMCs in a dose-dependent manner, detected by alizarin red staining and calcium content assay. Similarly, ALP activity and expression of bone-related molecules including Runx2, BMP2, and Osterix were also decreased in VSMCs treated with curcumin. In addition, flow cytometry analysis and caspase-3 activity assay revealed that curcumin treatment significantly suppressed apoptosis of VSMCs, which plays an important role during vascular calcification. Furthermore, we found that pro-apoptotic molecules including p-JNK and Bax were up-regulated in VSMCs treated with calcifying medium, but they were reduced in VSMCs after curcumin treatment. However, curcumin treatment has no effect on expression of NF-kappa B p65. Taken together, these findings suggest that curcumin attenuates apoptosis and calcification of VSMCs, presumably via inhibition of JNK/Bax signaling pathway.
引用
收藏
页码:151 / 160
页数:10
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