Identification and Functional Expression of the Mitochondrial Pyruvate Carrier

被引：561

作者：

Herzig, Sebastien ^{[1
]}

Raemy, Etienne ^{[1
]}

Montessuit, Sylvie ^{[1
]}

Veuthey, Jean-Luc ^{[2
]}

Zamboni, Nicola ^{[3
]}

Westermann, Benedikt ^{[4
]}

Kunji, Edmund R. S. ^{[5
]}

Martinou, Jean-Claude ^{[1
]}

机构：

[1] Univ Geneva, Dept Cell Biol, CH-1211 Geneva 4, Switzerland

[2] Univ Geneva, Sch Pharmaceut Sci, CH-1211 Geneva 4, Switzerland

[3] Inst Mol Syst Biol, CH-8093 Zurich, Switzerland

[4] Univ Bayreuth, D-95440 Bayreuth, Germany

[5] MRC, Mitochondria Biol Unit, Cambridge CB2 0XY, England

来源：

SCIENCE | 2012年 / 337卷 / 6090期

基金：

瑞士国家科学基金会;

关键词：

SACCHAROMYCES-CEREVISIAE; LIPOIC ACID; LACTOCOCCUS-LACTIS; DEHYDROGENASE; SPECIFICITY; CATABOLISM; TRANSPORT; MEMBRANE;

D O I：

10.1126/science.1218530

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The transport of pyruvate, the end product of glycolysis, into mitochondria is an essential process that provides the organelle with a major oxidative fuel. Although the existence of a specific mitochondrial pyruvate carrier (MPC) has been anticipated, its molecular identity remained unknown. We report that MPC is a heterocomplex formed by two members of a family of previously uncharacterized membrane proteins that are conserved from yeast to mammals. Members of the MPC family were found in the inner mitochondrial membrane, and yeast mutants lacking MPC proteins showed severe defects in mitochondrial pyruvate uptake. Coexpression of mouse MPC1 and MPC2 in Lactococcus lactis promoted transport of pyruvate across the membrane. These observations firmly establish these proteins as essential components of the MPC.

引用

页码：93 / 96

页数：4

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