Signal Amplification Circuit Composed of Serinol Nucleic Acid for RNA Detection

被引：2
|
作者：
Chen, Yanglingzhi [1 ]
Murayama, Keiji [1 ]
Asanuma, Hiroyuki [1 ]
机构：
[1] Nagoya Univ, Grad Sch Engn, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648603, Japan
来源：
CHEMISTRY LETTERS | 2022年 / 51卷 / 03期
关键词：
Acyclic nucleic acid; Hybridization chain reaction; RNA detection; HYBRIDIZATION CHAIN-REACTION; MESSENGER-RNA; DESIGN;
D O I：
10.1246/cl.210813
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A hybridization chain reaction (HCR) using a serinol nucleic acid (SNA) scaffold was newly designed and optimized. We found that hairpins with 8- or 9-mer loops, toeholds, and stems activated HCR and that the SNA interface accelerated initiation of HCR. Use of nitromethyl red as a quencher on the hairpin enabled detection of target RNA with high sensitivity. This system will be applicable to RNA detection in cell and biopsy, due to the high enzymatic durability of SNA.
引用
收藏
页码：330 / 333
页数：4
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