Genes for two multicopper proteins required for Fe(III) oxide reduction in Geobacter sulfurreducens have different expression patterns both in the subsurface and on energy-harvesting electrodes

被引:62
作者
Holmes, Dawn E. [1 ]
Mester, Tonde [1 ]
O'Neil, Regina A. [1 ]
Perpetua, Lorrie A. [1 ]
Larrahondo, M. Juliana [1 ]
Glaven, Richard [1 ]
Sharma, Manju L. [1 ]
Ward, Joy E. [1 ]
Nevin, Kelly P. [1 ]
Lovley, Derek R. [1 ]
机构
[1] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA
来源
MICROBIOLOGY-SGM | 2008年 / 154卷
关键词
D O I
10.1099/mic.0.2007/014365-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Previous studies have shown that Geobacter sulfurreducens requires the outer-membrane, multicopper protein OmpB for Fe(III) oxide reduction. A homologue of OmpB, designated OmpC, which is 36% similar to OmpB, has been discovered in the G. sulfurreducens genome. Deletion of ompC inhibited reduction of insoluble, but not soluble Fe(Ill). Analysis of multiple Geobacter and Pelobacter genomes, as well as in situ Geobacter, indicated that genes encoding multicopper proteins are conserved in Geobacter species but are not found in Pelobacter species. Levels of ompB transcripts were similar in G. sulfurreducens at different growth rates in chemostats and during growth on a microbial fuel cell anode. In contrast, ompC transcript levels increased at higher growth rates in chemostats and with increasing current production in fuel cells. Constant levels of Geobacter ompB transcripts were detected in groundwater during a field experiment in which acetate was added to the subsurface to promote in situ uranium bioremediation. In contrast, ompC transcript levels increased during the rapid phase of growth of Geobacter species following addition of acetate to the groundwater and then rapidly declined. These results demonstrate that more than one multicopper protein is required for optimal Fe(III) oxide reduction in G. sulfurreducens and suggest that, in environmental studies, quantifying OmpB/OmpC-related genes could help alleviate the problem that Pelobacter genes may be inadvertently quantified via quantitative analysis of 16S rRNA genes. Furthermore, comparison of differential expression of ompB and ompC may provide insight into the in situ metabolic state of Geobacter species in environments of interest.
引用
收藏
页码:1422 / 1435
页数:14
相关论文
共 85 条
[51]  
Lovley DR, 2006, NAT REV MICROBIOL, V4, P497, DOI 10.1038/nrmicro1442
[52]  
Lovley Derek R., 2002, OMICS A Journal of Integrative Biology, V6, P331, DOI 10.1089/153623102321112755
[53]   Cleaning up with genomics: Applying molecular biology to bioremediation [J].
Lovley, DR .
NATURE REVIEWS MICROBIOLOGY, 2003, 1 (01) :35-44
[54]   FE(III) AND S-0 REDUCTION BY PELOBACTER-CARBINOLICUS [J].
LOVLEY, DR ;
PHILLIPS, EJP ;
LONERGAN, DJ ;
WIDMAN, PK .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (06) :2132-2138
[55]   Dissimilatory Fe(III) and Mn(IV) reduction [J].
Lovley, DR ;
Holmes, DE ;
Nevin, KP .
ADVANCES IN MICROBIAL PHYSIOLOGY, VOL. 49, 2004, 49 :219-286
[56]   Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat [J].
Martins, LO ;
Soares, CM ;
Pereira, MM ;
Teixeira, M ;
Costa, T ;
Jones, GH ;
Henriques, AO .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (21) :18849-18859
[57]   Outer membrane c-type cytochromes required for Fe(III) and Mn(IV) oxide reduction in Geobacter sulfurreducens [J].
Mehta, T ;
Coppi, MV ;
Childers, SE ;
Lovley, DR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (12) :8634-8641
[58]   A putative multicopper protein secreted by an atypical type II secretion system involved in the reduction of insoluble electron acceptors in Geobacter sulfurreducens [J].
Mehta, Teena ;
Childers, Susan E. ;
Glaven, Richard ;
Lovley, Derek R. ;
Mester, Tunde .
MICROBIOLOGY-SGM, 2006, 152 :2257-2264
[59]   Genome of Geobacter sulfurreducens:: Metal reduction in subsurface environments [J].
Methé, BA ;
Nelson, KE ;
Eisen, JA ;
Paulsen, IT ;
Nelson, W ;
Heidelberg, JF ;
Wu, D ;
Wu, M ;
Ward, N ;
Beanan, MJ ;
Dodson, RJ ;
Madupu, R ;
Brinkac, LM ;
Daugherty, SC ;
DeBoy, RT ;
Durkin, AS ;
Gwinn, M ;
Kolonay, JF ;
Sullivan, SA ;
Haft, DH ;
Selengut, J ;
Davidsen, TM ;
Zafar, N ;
White, O ;
Tran, B ;
Romero, C ;
Forberger, HA ;
Weidman, J ;
Khouri, H ;
Feldblyum, TV ;
Utterback, TR ;
Van Aken, SE ;
Lovley, DR ;
Fraser, CM .
SCIENCE, 2003, 302 (5652) :1967-1969
[60]   Manganese(IV) oxide production by Acremonium sp strain KR21-2 and extracellular Mn(II) oxidase activity [J].
Miyata, Naoyuki ;
Tani, Yukinori ;
Maruo, Kanako ;
Tsuno, Hiroshi ;
Sakata, Masahiro ;
Iwahori, Keisuke .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (10) :6467-6473