Investigating immunomodulating activities of recombinant horse IL-2, IL-18 and IFN-γ in peripheral blood mononuclear cells (PBMCs)

被引：1
作者：
Saini, Sheetal [1 ,2 ]
Singha, Harisankar [1 ]
Siwach, Priyanka [2 ]
Tripathi, Bhupendra Nath [1 ]
机构：
[1] ICAR Natl Res Ctr Equines, Hisar 125001, Haryana, India
[2] Chaudhary Devi Lal Univ, Dept Biotechnol, Sirsa 125055, Haryana, India
来源：
INDIAN JOURNAL OF ANIMAL RESEARCH | 2020年 / 54卷 / 05期
关键词：
Cell proliferation; Cytokines; ELISA; Real time PCR; CYTOKINE MESSENGER-RNA; GENE-EXPRESSION; HUMAN INTERLEUKIN-2; CLONING; PURIFICATION; QUANTITATION; TH1;
D O I：
10.18805/ijar.B-3818
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Recombinant horse cytokines may be used as biological adjuvant for enhancing vaccine efficacy and therapeutic agent. This study describes low cost production of recombinant horse IL-2, IL-18 and IFN-gamma in prokaryotic system. lmmunomodulatory activities of recombinant horse IL-2, IL-18 and IFN-gamma have been demonstrated in horse peripheral blood mononuclear cells (PBMCs). Recombinant horse IL-2 and IL-18 enhanced cellular proliferation and significantly up regulated transcription and secretion of IFN-gamma, TNF-alpha, IL-4, IL-10 and IL-12p35. In contrast, recombinant horse IFN-gamma was comparatively less active and did not show significant immune-stimulatory effect.
引用
收藏
页码：601 / 607
页数：7
相关论文
共 32 条
  • [1] Expression of biologically active recombinant equine interferon-γ in Escherichia coli
    Bai, Yu
    Tong, Tiegang
    Liu, Guangliang
    Chen, Weiye
    Zhang, Weijun
    Wang, Qun
    Yang, Tao
    Bu, Zhigao
    Wu, Donglai
    [J]. COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 2010, 33 (04) : 333 - 342
  • [2] Effects of priming with cytokines on intracellular survival and replication of Rhodococcus equi in equine macrophages
    Berghaus, Londa J.
    Giguere, Steeve
    Bordin, Angela I.
    Cohen, Noah D.
    [J]. CYTOKINE, 2018, 102 : 7 - 11
  • [3] Quantitation of ovine cytokine mRNA by real-time RT-PCR
    Budhia, S
    Haring, LF
    McConnell, I
    Blacklaws, BA
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2006, 309 (1-2) : 160 - 172
  • [4] Interleukin 2 plays a central role in Th2 differentiation
    Cote-Sierra, J
    Foucras, G
    Guo, LY
    Chiodetti, L
    Young, HA
    Hu-Li, J
    Zhu, JF
    Paul, WE
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (11) : 3880 - 3885
  • [5] Expression and characterisation of equine interleukin 2 and interleukin 4
    Dohmann, K
    Wagner, B
    Horohov, DW
    Leibold, W
    [J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2000, 77 (3-4) : 243 - 256
  • [6] Validation of a reliable set of primer pairs for measuring gene expression by real-time quantitative RT-PCR in equine leukocytes
    Figueiredo, M. D.
    Salter, C. E.
    Andrietti, A. L. P.
    Vandenplas, M. L.
    Hurley, D. J.
    Moore, J. N.
    [J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2009, 131 (1-2) : 65 - 72
  • [7] Quantitation of rabbit cytokine mRNA by real-time RT-PCR
    Godornes, Charmie
    Leader, Brandon Troy
    Molini, Barbara J.
    Centurion-Lara, Arturo
    Lukehart, Sheila A.
    [J]. CYTOKINE, 2007, 38 (01) : 1 - 7
  • [8] JU G, 1987, J BIOL CHEM, V262, P5723
  • [9] Cytokine gene expression by peripheral blood leukocytes in horses experimentally infected with Anaplasma phagocytophila
    Kim, HY
    Mott, J
    Zhi, N
    Tajima, T
    Rikihisa, Y
    [J]. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2002, 9 (05) : 1079 - 1084
  • [10] The pathogenic and vaccine strains of equine infectious anemia virus differentially induce cytokine and chemokine expression and apoptosis in macrophages
    Lin, Yue-Zhi
    Cao, Xue-Zhi
    Li, Liang
    Li, Li
    Jiang, Cheng-Gang
    Wang, Xue-Feng
    Ma, Jian
    Zhou, Jian-Hua
    [J]. VIRUS RESEARCH, 2011, 160 (1-2) : 274 - 282
1234