Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides and Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice

被引:23
作者
Fu, Xiaodan [1 ]
Zhan, Yuming [2 ]
Li, Nannan [3 ]
Yu, Dongxing [4 ]
Gao, Wei [3 ]
Gu, Ziqiang [3 ]
Zhu, Lin [3 ]
Li, Rong [5 ]
Zhu, Changliang [3 ]
机构
[1] Nanchang Univ, Key Lab Bioact Polysaccharides Jiangxi Prov, China Canada Joint Lab Food Sci & Technol Nanchan, State Key Lab Food Sci & Technol, Nanchang, Peoples R China
[2] Shandong Feed & Vet Drug Qual Ctr, Jinan, Peoples R China
[3] Ocean Univ China, Coll Food Sci & Engn, Qingdao, Peoples R China
[4] SOHAO FD TECH CO LTD, Qingdao, Peoples R China
[5] Qingdao Women & Childrens Hosp, Qingdao, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
Laminaria japonica; cellulase; alginate lyase; low-molecular-weight polysaccharides; high-fat diet; intestinal microbiota; GUT MICROBIOTA; AKKERMANSIA-MUCINIPHILA; ACIDS; OBESITY; CARBOHYDRATE; DEGRADATION;
D O I
10.3389/fbioe.2021.820892
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Recent studies have shown that seaweed polysaccharides can ameliorate high-fat-diet (HFD)-induced metabolic syndromes associated with the regulatory function of gut microbiota. However, kelp, a natural source of seaweed polysaccharides, is highly viscous, making it difficult to prepare dietary fiber by simple degradation. Therefore, we developed a novel method of preparing low-molecular-weight polysaccharides from Laminaria japonica by combining high-pressure pretreatment and composite enzymatic degradation and evaluated the obesity prevention activity of these polysaccharides. Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate. The in vivo effects of SJP on the intestinal microbiota were also investigated using HFD-fed C57BL/6J mice. SJP reduced weight gain and fat deposition in HFD-fed mice and increased the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces. SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus. Further, SJP enhanced the abundance of Akkermansia muciniphila in mice provided with HFD and normal chow. Single-strain culture experiments also revealed that SJP promoted the growth of A. muciniphila. This study highlights the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals.
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页数:16
相关论文
共 45 条
[1]   Hyaluronic acid and Chondroitin sulfate from marine and terrestrial sources: Extraction and purification methods [J].
Abdallah, Maha M. ;
Fernandez, Naiara ;
Matias, Ana A. ;
Bronze, Maria do Rosario .
CARBOHYDRATE POLYMERS, 2020, 243
[2]   Perspective: Physiologic Importance of Short-Chain Fatty Acids from Nondigestible Carbohydrate Fermentation [J].
Alexander, Celeste ;
Swanson, Kelly S. ;
Fahey, George C., Jr. ;
Garleb, Keith A. .
ADVANCES IN NUTRITION, 2019, 10 (04) :576-589
[3]  
[Anonymous], 2015, Official methods of analysis of AOAC Int, V19th
[4]   Phylotype-level 16S rRNA analysis reveals new bacterial indicators of health state in acute murine colitis [J].
Berry, David ;
Schwab, Clarissa ;
Milinovich, Gabriel ;
Reichert, Jochen ;
Ben Mahfoudh, Karim ;
Decker, Thomas ;
Engel, Marion ;
Hai, Brigitte ;
Hainzl, Eva ;
Heider, Susanne ;
Kenner, Lukas ;
Mueller, Mathias ;
Rauch, Isabella ;
Strobl, Birgit ;
Wagner, Michael ;
Schleper, Christa ;
Urich, Tim ;
Loy, Alexander .
ISME JOURNAL, 2012, 6 (11) :2091-2106
[5]   Obesity: global epidemiology and pathogenesis [J].
Blueher, Matthias .
NATURE REVIEWS ENDOCRINOLOGY, 2019, 15 (05) :288-298
[6]   NEW METHOD FOR QUANTITATIVE-DETERMINATION OF URONIC ACIDS [J].
BLUMENKR.N ;
ASBOEHAN.G .
ANALYTICAL BIOCHEMISTRY, 1973, 54 (02) :484-489
[7]   Polysaccharides from Laminaria japonica: Structural characteristics and antioxidant activity [J].
Cui, Chun ;
Lu, Jianghong ;
Sun-Waterhouse, Dongxiao ;
Mu, Lixia ;
Sun, Weizheng ;
Zhao, Mouming ;
Zhao, Haifeng .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2016, 73 :602-608
[8]   The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism [J].
den Besten, Gijs ;
van Eunen, Karen ;
Groen, Albert K. ;
Venema, Koen ;
Reijngoud, Dirk-Jan ;
Bakker, Barbara M. .
JOURNAL OF LIPID RESEARCH, 2013, 54 (09) :2325-2340
[9]   Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides [J].
Dobrincic, Ana ;
Balbino, Sandra ;
Zoric, Zoran ;
Pedisic, Sandra ;
Kovacevic, Danijela Bursac ;
Garofulic, Ivona Elez ;
Dragovic-Uzelac, Verica .
MARINE DRUGS, 2020, 18 (03)