Discovery and Genomic Characterization of a Novel Henipavirus, Angavokely Virus, from Fruit Bats in Madagascar

被引:33
|
作者
Madera, Sharline [1 ]
Kistler, Amy [2 ]
Ranaivoson, Hafaliana C. [3 ,4 ,5 ]
Ahyong, Vida [2 ]
Andrianiaina, Angelo [5 ]
Andry, Santino [6 ]
Raharinosy, Vololoniaina [4 ]
Randriambolamanantsoa, Tsiry H. [4 ]
Ravelomanantsoa, Ny Anjara Fifi [5 ]
Tato, Cristina M. [2 ]
DeRisi, Joseph L. [2 ,7 ]
Aguilar, Hector C. [8 ]
Lacoste, Vincent [4 ]
Dussart, Philippe [4 ]
Heraud, Jean-Michel [4 ,9 ]
Brook, Cara E. [3 ]
机构
[1] Univ Calif San Francisco, Dept Med, Div Infect Dis, San Francisco, CA 94143 USA
[2] Chan Zuckerberg Biohub, San Francisco, CA USA
[3] Univ Chicago, Dept Ecol & Evolut, 940 E 57Th St, Chicago, IL 60637 USA
[4] Inst Pasteur Madagascar, Virol Unit, Antananarivo, Madagascar
[5] Univ Antananarivo, Dept Zool & Anim Biodivers, Antananarivo, Madagascar
[6] Univ Antananarivo, Dept Entomol, Antananarivo, Madagascar
[7] Univ Calif San Francisco, Dept Med & Biochem & Biophys, San Francisco, CA 94143 USA
[8] Cornell Univ, Coll Vet Med, Dept Microbiol & Immunol, Ithaca, NY 14853 USA
[9] Inst Pasteur, Virol Dept, Dakar, Senegal
基金
美国国家卫生研究院;
关键词
emerging zoonosis; henipavirus; novel virus; Eidolon dupreanum; bat-borne virus; Madagascar; NIPAH-VIRUS; ALIGNMENT; HENDRA; EPHRINB2; RECEPTOR; DISEASE;
D O I
10.1128/jvi.00921-22
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Henipaviruses include highly pathogenic emerging zoonotic viruses, derived from bat, rodent, and shrew reservoirs. Bat-borne Hendra (HeV) and Nipah (NiV) are the most well-known henipaviruses, for which no effective antivirals or vaccines for humans have been described. The genus Henipavirus (family Paramyxoviridae) currently comprises seven viruses, four of which have demonstrated prior evidence of zoonotic capacity. These include the biosafety level 4 agents Hendra (HeV) and Nipah (NiV) viruses, which circulate naturally in pteropodid fruit bats. Here, we describe and characterize Angavokely virus (AngV), a divergent henipavirus identified in urine samples from wild, Madagascar fruit bats. We report the nearly complete 16,740-nucleotide genome of AngV, which encodes the six major henipavirus structural proteins (nucleocapsid, phosphoprotein, matrix, fusion, glycoprotein, and L polymerase). Within the phosphoprotein (P) gene, we identify an alternative start codon encoding the AngV C protein and a putative mRNA editing site where the insertion of one or two guanine residues encodes, respectively, additional V and W proteins. In other paramyxovirus systems, C, V, and W are accessory proteins involved in antagonism of host immune responses during infection. Phylogenetic analysis suggests that AngV is ancestral to all four previously described bat henipaviruses-HeV, NiV, Cedar virus (CedV), and Ghanaian bat virus (GhV)-but evolved more recently than rodent- and shrew-derived henipaviruses, Mojiang (MojV), Gamak (GAKV), and Daeryong (DARV) viruses. Predictive structure-based alignments suggest that AngV is unlikely to bind ephrin receptors, which mediate cell entry for all other known bat henipaviruses. Identification of the AngV receptor is needed to clarify the virus's potential host range. The presence of V and W proteins in the AngV genome suggest that the virus could be pathogenic following zoonotic spillover. IMPORTANCE Henipaviruses include highly pathogenic emerging zoonotic viruses, derived from bat, rodent, and shrew reservoirs. Bat-borne Hendra (HeV) and Nipah (NiV) are the most well-known henipaviruses, for which no effective antivirals or vaccines for humans have been described. Here, we report the discovery and characterization of a novel henipavirus, Angavokely virus (AngV), isolated from wild fruit bats in Madagascar. Genomic characterization of AngV reveals all major features associated with pathogenicity in other henipaviruses, suggesting that AngV could be pathogenic following spillover to human hosts. Our work suggests that AngV is an ancestral bat henipavirus that likely uses viral entry pathways distinct from those previously described for HeV and NiV. In Madagascar, bats are consumed as a source of human food, presenting opportunities for cross-species transmission. Characterization of novel henipaviruses and documentation of their pathogenic and zoonotic potential are essential to predicting and preventing the emergence of future zoonoses that cause pandemics.
引用
收藏
页数:13
相关论文
共 50 条
  • [21] Genomic Characterization of a Novel Virus of the Family Tymoviridae Isolated from Mosquitoes
    Wang, Lihua
    Lv, Xinjun
    Zhai, Yougang
    Fu, Shihong
    Wang, David
    Rayner, Simon
    Tang, Qing
    Liang, Guodong
    PLOS ONE, 2012, 7 (07):
  • [22] Genomic characterization of Alphacoronavirus from Mops condylurus bats in Nigeria
    George, Uwem
    George, Oluwadamilola
    Oguzie, Judith
    Osasona, Oluwadamilola
    Motayo, Babatunde
    Kamani, Joshua
    Eromon, Philomena
    Folarin, Onikepe
    Happi, Anise
    Komolafe, Isaac
    Happi, Christian
    VIRUS RESEARCH, 2023, 334
  • [23] Molecular detection of a novel paramyxovirus in fruit bats from Indonesia
    Sasaki, Michihito
    Setiyono, Agus
    Handharyani, Ekowati
    Rahmadani, Ibenu
    Taha, Siswatiana
    Adiani, Sri
    Subangkit, Mawar
    Sawa, Hirofumi
    Nakamura, Ichiro
    Kimura, Takashi
    VIROLOGY JOURNAL, 2012, 9
  • [24] Molecular detection of a novel paramyxovirus in fruit bats from Indonesia
    Michihito Sasaki
    Agus Setiyono
    Ekowati Handharyani
    Ibenu Rahmadani
    Siswatiana Taha
    Sri Adiani
    Mawar Subangkit
    Hirofumi Sawa
    Ichiro Nakamura
    Takashi Kimura
    Virology Journal, 9
  • [25] Genomic characterization and infectivity of a novel SARS-like coronavirus in Chinese bats
    Hu, Dan
    Zhu, Changqiang
    Ai, Lele
    He, Ting
    Wang, Yi
    Ye, Fuqiang
    Yang, Lu
    Ding, Chenxi
    Zhu, Xuhui
    Lv, Ruicheng
    Zhu, Jin
    Hassan, Bachar
    Feng, Youjun
    Tan, Weilong
    Wang, Changjun
    EMERGING MICROBES & INFECTIONS, 2018, 7
  • [26] Discovery and Genomic Characterization of a Novel Hepadnavirus from Asymptomatic Anadromous Alewife (Alosa pseudoharengus)
    Raines, Clayton
    Lovy, Jan
    Phelps, Nicolas
    Mor, Sunil
    Ng, Terry Fei Fan
    Iwanowicz, Luke
    VIRUSES-BASEL, 2024, 16 (06):
  • [27] EXPERIMENTAL INFECTION OF JAMAICAN FRUIT BATS (ARTIBEUS JAMAICENSIS) WITH BUKAKATA ORBIVIRUS, A NOVEL VIRUS FROM A UGANDAN BAT
    Fagre, Anna C.
    Byas, Alex
    Malmlov, Ashley
    Bergren, Nicholas
    Borland, Erin M.
    Rice, Lauren
    Schountz, Tony
    Kading, Rebekah
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2019, 101 : 262 - 262
  • [28] ISOLATION AND CHARACTERIZATION OF BUKAKATA ORBIVIRUS, A NOVEL VIRUS FROM A UGANDAN BAT, AND ASSOCIATED PULMONARY PATHOLOGY IN EXPERIMENTALLY INFECTED JAMAICAN FRUIT BATS (ARTIBEUS JAMAICENSIS)
    Fagre, Anna C.
    Kityo, Robert
    Lee, Justin
    Mossel, Eric
    Crabtree, Mary
    Nalikka, Betty
    Nakayiki, Teddie
    Kerbis, Julian
    Gilbert, Amy
    Bergren, Nicholas
    Nyakarahuka, Luke
    Lutwama, Julius
    Stenglein, Mark
    Byas, Alexandria
    Malmlov, Ashley
    Rice, Lauren
    Miller, Barry
    Schountz, Tony
    Kading, Rebekah
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2017, 97 (05): : 50 - 50
  • [29] A novel cassava-infecting begomovirus from Madagascar: cassava mosaic Madagascar virus
    Mireille Harimalala
    Pierre Lefeuvre
    Alexandre De Bruyn
    Fidèle Tiendrébéogo
    Murielle Hoareau
    Julie Villemot
    Sahondramalala Ranomenjanahary
    Alice Andrianjaka
    Bernard Reynaud
    Jean-Michel Lett
    Archives of Virology, 2012, 157 : 2027 - 2030
  • [30] A novel cassava-infecting begomovirus from Madagascar: cassava mosaic Madagascar virus
    Harimalala, Mireille
    Lefeuvre, Pierre
    De Bruyn, Alexandre
    Tiendrebeogo, Fidele
    Hoareau, Murielle
    Villemot, Julie
    Ranomenjanahary, Sahondramalala
    Andrianjaka, Alice
    Reynaud, Bernard
    Lett, Jean-Michel
    ARCHIVES OF VIROLOGY, 2012, 157 (10) : 2027 - 2030