Metabolic alteration of urinary steroids in pre- and post-menopausal women, and men with papillary thyroid carcinoma

被引:23
作者
Choi, Man Ho [1 ]
Moon, Ju-Yeon [1 ]
Cho, Sung-Hee [1 ]
Chung, Bong Chul [1 ]
Lee, Eun Jig [2 ]
机构
[1] Korea Inst Sci & Technol, Future Convergence Res Div, Seoul 136791, South Korea
[2] Yonsei Univ, Coll Med, Dept Internal Med, Seoul 120752, South Korea
关键词
Steroids; Thyroid cancer; Menopause; Gender difference; GC-MS; ESTROGEN-RECEPTOR; MOLECULAR MECHANISMS; ANDROGEN RECEPTORS; POOLED ANALYSIS; BREAST-CANCER; DNA-DAMAGE; CELLS; GROWTH; TUMORS; RISK;
D O I
10.1186/1471-2407-11-342
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: To evaluate the metabolic changes in urinary steroids in pre- and post-menopausal women and men with papillary thyroid carcinoma (PTC). Methods: Quantitative steroid profiling combined with gas chromatography-mass spectrometry was used to measure the urinary concentrations of 84 steroids in both pre- (n = 21, age: 36.95 +/- 7.19 yr) and post-menopausal female (n = 19, age: 52.79 +/- 7.66 yr), and male (n = 16, age: 41.88 +/- 8.48 yr) patients with PTC. After comparing the quantitative data of the patients with their corresponding controls (pre-menopause women: n = 24, age: 33.21 +/- 10.48 yr, post-menopause women: n = 16, age: 49.67 +/- 8.94 yr, male: n = 20, age: 42.75 +/- 4.22 yr), the levels of steroids in the patients were normalized to the mean concentration of the controls to exclude gender and menopausal variations. Results: Many urinary steroids were up-regulated in all PTC patients compared to the controls. Among them, the levels of three active androgens, androstenedione, androstenediol and 16 alpha-hydroxy DHEA, were significantly higher in the pre-menopausal women and men with PTC. The corticoid levels were increased slightly in the PTC men, while progestins were not altered in the post-menopausal PTC women. Estrogens were up-regulated in all PTC patients but 2-hydroxyestrone and 2-hydroxy-17 beta-estradiol were remarkably changed in both pre-menopausal women and men with PTC. For both menopausal and gender differences, the 2-hydroxylation, 4-hydroxylation, 2-methoxylation, and 4-methoxylation of estrogens and 16 alpha-hydroxylation of DHEA were differentiated between pre- and post-menopausal PTC women (P < 0.001). In particular, the metabolic ratio of 2-hydroxyestrone to 2-hydroxy-17 beta-estradiol, which could reveal the enzyme activity of 17 beta-hydroxysteroid dehydrogenase, showed gender differences in PTC patients (P < 1 x 10(-7)). Conclusions: These results are expected be helpful for better understanding the pathogenic differences in PTC according to gender and menopausal conditions.
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页数:14
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共 34 条
[1]   Testosterone and estradiol up-regulate androgen and estrogen receptors in immature and adult rat thyroid glands in vivo [J].
Banu, SK ;
Govindarajulu, P ;
Aruldhas, MM .
STEROIDS, 2002, 67 (13-14) :1007-1014
[2]   ROLE OF GLUCOCORTICOIDS IN THE REGULATION OF LIPOGENESIS [J].
BERDANIER, CD .
FASEB JOURNAL, 1989, 3 (10) :2179-2183
[3]  
Bradlow HI, 1996, J ENDOCRINOL, V150, pS259
[4]   A hormonal association between estrogen metabolism and proliferative thyroid disease [J].
Chan, EK ;
Sepkovic, DW ;
Bowne, HJY ;
Yu, GP ;
Schantz, SP .
OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 2006, 134 (06) :893-900
[5]   Quantitative metabolic profiling of 21 endogenous corticosteroids in urine by liquid chromatography-triple quadrupole-mass spectrometry [J].
Cho, Hye-Jin ;
Kim, Jong Dae ;
Lee, Won-Yong ;
Chung, Bong Chul ;
Choi, Man Ho .
ANALYTICA CHIMICA ACTA, 2009, 632 (01) :101-108
[6]  
CORREA P, 1995, CANCER, V75, P338, DOI 10.1002/1097-0142(19950101)75:1+<338::AID-CNCR2820751316>3.0.CO
[7]  
2-F
[8]  
DIAZ NM, 1991, ARCH PATHOL LAB MED, V115, P1203
[9]   PHYSIOLOGICAL AND MOLECULAR MECHANISMS INVOLVED IN NUTRITIONAL REGULATION OF FATTY-ACID SYNTHESIS [J].
HILLGARTNER, F ;
SALATI, LM ;
GOODRIDGE, AG .
PHYSIOLOGICAL REVIEWS, 1995, 75 (01) :47-76
[10]   2-hydroxyestradiol induces oxidative DNA damage and apoptosis in human mammary epithelial cells [J].
Hurh, YJ ;
Chen, ZH ;
Na, HK ;
Han, SY ;
Surh, YJ .
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, 2004, 67 (23-24) :1939-1953