New methodology for evaluating osteoclastic activity induced by orthodontic load

被引:16
作者
Araujo, Adriele Silveira [1 ]
Nolasco Fernandes, Alline Birra [1 ]
Bolognesi Maciel, Jose Vinicius [1 ]
Santos Netto, Juliana de Noronha [2 ]
Bolognese, Ana Maria [1 ]
机构
[1] Univ Fed Rio de Janeiro, Fac Odontol, Dept Ortodontia & Odontopediat, BR-21941617 Rio De Janeiro, RJ, Brazil
[2] Univ Fed Rio de Janeiro, Sch Dent, Dept Oral Pathol & Oral Diag, BR-21941617 Rio De Janeiro, Brazil
关键词
Tooth movement; Osteoclasts; Alendronate; Fractals; TOOTH MOVEMENT; FRACTAL ANALYSIS; RATS; BISPHOSPHONATES; IRREGULARITY; ALENDRONATE; MECHANISMS;
D O I
10.1590/1678-775720140351
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Orthodontic tooth movement (OTM) is a dynamic process of bone modeling involving osteoclast-driven resorption on the compression side. Consequently, to estimate the influence of various situations on tooth movement, experimental studies need to analyze this cell. Objectives: The aim of this study was to test and validate a new method for evaluating osteoclastic activity stimulated by mechanical loading based on the fractal analysis of the periodontal ligament (PDL)-bone interface. Material and Methods: The mandibular right first molars of 14 rabbits were tipped mesially by a coil spring exerting a constant force of 85 cN. To evaluate the actual influence of osteoclasts on fractal dimension of bone surface, alendronate (3 mg/Kg) was injected weekly in seven of those rabbits. After 21 days, the animals were killed and their jaws were processed for histological evaluation. Osteoclast counts and fractal analysis (by the box counting method) of the PDL-bone interface were performed in histological sections of the right and left sides of the mandible. Results: An increase in the number of osteoclasts and in fractal dimension after OTM only happened when alendronate was not administered. Strong correlation was found between the number of osteoclasts and fractal dimension. Conclusions: Our results suggest that osteoclastic activity leads to an increase in bone surface irregularity, which can be quantified by its fractal dimension. This makes fractal analysis by the box counting method a potential tool for the assessment of osteoclastic activity on bone surfaces in microscopic examination.
引用
收藏
页码:19 / 25
页数:7
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