Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability

被引：8

作者：

Suarez, Gianmarco D. ^{[1
,7
]}

Suarez, Domenick A. ^{[7
]}

Tang, Yuki Yu Kiu ^{[7
]}

Zhang, Jing-Xuan ^{[4
]}

Li, Jun ^{[4
,5
,6
]}

Nagl, Stefan ^{[1
]}

Cheung, Peter Pak-Hang ^{[2
,3
,7
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Chem, Clear Water Bay, Kowloon, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Prince Wales Hosp, Fac Med, Dept Chem Pathol,Shatin, Hong Kong, Peoples R China

[3] Chinese Univ Hong Kong, Prince Wales Hosp, Li Ka Shing Inst Hlth Sci, Shatin, Li Ka Shing Med Sci Bldg, Hong Kong, Peoples R China

[4] Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Guangdong, Peoples R China

[5] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China

[6] Tsinghua Univ, Key Lab Organ Optoelect & Mol Engn, Minist Educ, Beijing 100084, Peoples R China

[7] Quommni Technol Ltd, Hong Kong, Peoples R China

来源：

ANALYTICAL METHODS | 2022年 / 14卷 / 04期

关键词：

MEDIATED ISOTHERMAL AMPLIFICATION;

D O I：

10.1039/d1ay01395e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Improved diagnostics are needed to manage the ongoing COVID-19 pandemic. In this study, we enhanced the color changes and sensitivity of colorimetric SARS-CoV-2 RT-LAMP assays based on triarylmethane dyes. We determined a mechanism for the color changes and obtained sensitivities of 10 RNA copies per microliter.

引用

页码：378 / 382

页数：5

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